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Archive > Volume 29 Issue 10 > 2022,29(10):896-903. DOI:10.3872/j.issn.1007-385X.2022.10.005 Prev Next
Basic Research
BNC1 regulates the malignant biological behaviors of esophageal squamous cell carcinoma cells and its possible mechanism
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Abstract:
Objective: To investigate the effects of basonuclin 1 (BNC1) on the proliferation, migration, invasion, cell cycle and apoptosis of esophageal squamous cell carcinoma (ESCC) cells and its mechanism. Methods: TThe mRNA expression level of BNC1 in ESCC cells and normal esophageal epithelial cells was detected by qPCR. The protein expression level of BNC1 in cancer and para-cancerous tissues of 10 ESCC patients was detected by immunohistochemical staining. The expression of BNC1 in KYSE-150 and KYSE-30 cells was down-regulated by siRNA, and the effects of BNC1 on cell proliferation, migration, invasion, cell cycle andapoptosis were investigated using CCK-8, scratch healing, Transwell and flow cytometry assays, respectively. The downstream targetgenes of BNC1 were identified by ChIP-seq assay and GEPIA online website date analysis combined with knockdown transcriptome sequencing data after BNC1 knockdown. qPCR was used to verify the expression of target genes after BNC1 knockdown, and dualluciferase reporter gene assay was used to confirm the regulatory effect of BNC1 on the target genes. Results:The mRNA and protein levels of BNC1 were higher in ESCC tissues than in para-cancerous tissues (all P<0.01). Knockdown of BNC1 significantly inhibited the proliferation, migration and invasion of KYSE-150 and KYSE-30 cells (P<0.01 or P<0.01), arrested the cells in G1 phase and promoted the cell apoptosis (all P<0.01). The results of ChIP-seq assay and online website GEPIA date analysis combined with at transcriptome sequencing data indicated after BNC1 knockdown that G protein pathway repressor 1 (GPS1) may be a oncogenic target gene that positively regulated by BNC1. The results of qPCR and dual luciferase reporter gene assay showed that BNC1 had a regulatory effect on GPS1 (P<0.01). Conclusion: BNC1 is highly expressed in ESCC tissues and cells. Interfering with BNC1 significantly inhibits the proliferation, migration and invasion of ESCC cells, arrests cells at G1 phase and promotes cell apoptosis possibly by targeting GPS1 to regulate the malignant biological behaviors of ESCC cells.
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