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Archive > Volume 29 Issue 10 > 2022,29(10):904-910. DOI:10.3872/j.issn.1007-385X.2022.10.006 Prev Next
Clinical Research
Clinical significance of CCDC137 gene expression in hepatocellular carcinoma tissues and its effect on the proliferation, migration and invasion of MHCC97H cells
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Abstract:
Objective: To analyze the gene expression of coiled-coil domain containing protein 137 (CCDC137) in hepatocellular carcinoma (HCC) tissues and its relationship with clinicopathological features and prognosis of HCC patients, and to explore the effect of CCDC137 knockdown on the proliferation, migration and invasion of MHCC97H cells. Methods: The HCC dataset was downloaded from The Cancer Genome Atlas (TCGA) to obtain the CCDC137 gene expression profile and clinical information of patients. The correlation between the expression level of CCDC137 gene in HCC tissues and the clinicopathological indices and its impact on patients' prognosis were analyzed by bioinformatics method. Gene Set Enrichment Analysis (GSEA) was used to predict the possible pathways regulated by CCDC137 gene in HCC. Kaplan-Meier method and Log-Rank test were used for survival analysis; Cox proportional hazards regression model was used to analyze the risk factors affecting the prognosis of patients. The expression of CCDC137 in MHCC97H cells was inhibited by small interfering RNA technology. The mRNA and protein expression levels of CCDC137 in transfected MHCC97H cells as well as its effect on cell proliferation, migration and invasion were observed by qPCR,WB, CCK-8 and Transwell assays, respectively. Results: According to the data of 371 HCC patients retrieved from TCGA database, the expression level of CCDC137 mRNA in tumor tissues was significantly higher than that in the para-cancerous tissues (P<0.01). The high expression of CCDC137 mRNA was significantly correlated with tumor histological grade (OR=0.014), cancer tissue stage (OR=0.007), and T stage (OR=0.047) (all P<0.05). The OS rate of patients with high CCDC137 expression was significantly lower than that of patients with low CCDC137 expression (P<0.05), and multivariate Cox regression analysis suggested that CCDC137 gene could be an independent prognostic factor for HCC patients. GSEA results showed that the samples with high expression of CCDC137 gene were enriched in multiple pathways/gene sets such as base excision repair and spliceosome (P<0.01, FDR<0.05). After knockdown of CCDC137 gene, the proliferation, migration and invasion of MHCC97H cells were significantly decreased (all P<0.01).Conclusion: CCDC137 gene is highly expressed in HCC tissues. The high expression of CCDC137 is related to the occurrence,development and poor prognosis of HCC. Inhibition of CCDC137 gene expression can inhibit the proliferation, migration and invasion of MHCC97H cells.
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