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Archive > Volume 29 Issue 12 > 2022,29(12):1087-1093. DOI:10.3872/j.issn.1007-385X.2022.12.004 Prev Next
Basic Research
Clinical significance of FOXD1 expression in esophageal squamous cell carcinoma and the mechanism of its effect on the malignant biological behaviors of TE1 cells
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Abstract:
Objective: To analyze the expression of FOXD1 in esophageal squamous cell carcinoma (ESCC) and its relationship with clinicopathological features and prognosis. To investigate the effect of FOXD1 on the proliferation and invasion of TE1 cells and its effect on TGF- β1 induction of epithelial-mesenchymal transition (EMT). Methods: The expression of FOXD1 in ESCC tissues and cells was detected by qPCR and immunohistochemical method (IHC). The relationships between its expression level and clinicopathological characteristics and prognosis of patients were also analyzed. FOXD1 knockdown plasmid was constructed and transfected into TE1 cells to detect its effect on the proliferation and invasion of TE1 cells. qPCR and Western blotting were used to detect the expression levels of FOXD1 and EMT-related markers before and after TGF- β1 treatment and the effect of knockdown FOXD1 on the expression of EMT-related markers. Results: FOXD1 was highly expressed in ESCC tissues and cells (all P<0.01), and negatively correlated with the overall survival of patients. FOXD1 expression level, tumor TNM stage, and lymph node metastasis were independent factors affecting the prognosis of ESCC patients (all P<0.01). TGF-β1 treatment may raise the expression level of FOXD1 in TE1 cells and induce the expression of EMT-related markers (all P<0.05). Knockdown FOXD1 may suppress the proliferation and invasion ability of TE1 cells and can partially reverse the EMT process of TE1 cells induced by TGF-β1. Conclusion: FOXD1, highly expressed in ESCC tissues and TE1 cells, is an independent factor affecting the prognosis of ESCC patients. Knockdown of FOXD1 can significantly inhibit the proliferation, invasion and TGF-β1 mediated EMT process of TE1 cells.
Keywords:
esophageal squamous cell carcinoma (ESCC) ; FOXD1 ; TGF- β1 ; proliferation ; invasion ; epithelial mesenchymal transition (EMT) ; prognosis
