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Archive > Volume 29 Issue 12 > 2022,29(12):1101-1107. DOI:10.3872/j.issn.1007-385X.2022.12.006 Prev Next
Basic Research
The effects of circFBXL5 on the malignant biological behaviors of bladder cancer T24 cells by targeting miR-515-5p and its molecular mechanisms
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Abstract:
Objective: To investigate the effects of circFBXL5 on the proliferation, migration, and invasion of bladder cancer T24 cells by targeting miR-515-5p and its possible molecular mechanisms. Methods: 41 bladder cancer tissues and their adjacent tissues were collected at Suzhou Hospital of Integrated Traditional Chinese and Western Medicine from April 2020 to June 2020. The expressions of circFBXL5 and miR-515-5p were detected by qPCR. Dual-luciferase reporter assay was used to verify the targeting relationship between circFBXL5 and miR-515-5p. Human bladder cancer cells T24 were cultured in vitro and divided into si-NC group, si-circFBXL5 group, anti-miR-NC+si-circFBXL5 group, and si-circFBXL5+anti-miR-515-5p group. MTT assay, plate colony formation assay, flow cytometry, Transwell assay and Western blotting were used to detect cell proliferation, clone formation, migration, invasion, apoptosis and BAX and Bcl-2 protein levels of T24 cells after transfection, respectively. Results: circFBXL5 was highly expressed and miR-515-5p was lowly expressed in bladder cancer tissues (all P<0.05). circFBXL5 could negatively regulate the expression of miR-515-5p. After the knockdown of circFBXL5, the cell proliferation inhibition rate, apoptosis rate, and protein level of BAX were significantly increased (all P<0.05); the number of cell clone formation, migration, and invasion cells were decreased (all P<0.05); the protein level of Bcl-2 was significantly decreased (P <0.05). Knockdown of circFBXL5 and miR-515-5p simultaneously could partially reverse the inhibiting effect of circFBXL5 knockdown on T24 cells. Conclusion: circFBXL5 promoted the proliferation, migration, and invasion of bladder cancer T24 cells by inhibiting miR-515-5p expression. circFBXL5 and miR-515-5p may serve as potential molecular targets for bladder cancer treatment.
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