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Clinical Research
Analysis of the value of NFKBIA in prognosis and immune infiltration of SKCM based on multiple public databases
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Abstract:
Objective: To evaluate the association between the expression of NF-kB inhibitor alpha (NFKBIA) gene and the prognosis and immune infiltration of the tumor microenvironment in patients with skin cutaneous melanoma (SKCM). Methods: GEPIA2 database was used to analyze the differential expression of NFKBIA in SKCM tissues and normal skin tissues. GEPIA2 and Ualcan databases were utilized to analyze the association between NFKBIA expression and SKCM prognosis. TIMER and TISIDB were used to investigate the correlation between NFKBIA and tumor-infiltrating lymphocytes (TIL) and immune regulator genes in SKCM. The association between NFKBIA and subsets of SKCM cells as well as their functional states were analyzed at single-cell level in TISCH and Cancer SEA databases. The paraffin embedded tissues from 14 SKCM patients preserved in Jingmen No.2 People′s Hospital were obtained for this study, and immunohistochemical staining was used to detect the NFKBIA protein expression in SKCM tissues and para-cancerous tissues. Results: NFKBIA was lowly expressed in SKCM tissues, and SKCM patients with low NFKBIA expression had a poor prognosis (P<0.05). NFKBIA expression level was positively correlated with the infiltration of B cells, CD8+ T cells, CD4+ T cells, macrophages, neutrophils and dendritic cells (all P<0.01). What's more, the expression of NFKBIA was positively correlated with TIL abundance and immunoregulatory genes (all P<0.01). NFKBIA was expressed in SKCM immune cells and positively correlated with cell differentiation and inflammation in tumor microenvironment (R=0.28, 0.23, all P<0.05). Immunohistochemical staining results demonstrated that the protein expression of NFKBIA was significantly lower in SKCM tissues than that in para-cancerous tissues (35.71% vs 85.71%, P<0.05). Conclusions: NFKBIA has a low expression in SKCM tissues, and it is correlated with immune infiltration in SKCM, which can be used as a prognostic marker and treatment target for SKCM.
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