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Archive > Volume 30 Issue 2 > 2023,30(2):142-149. DOI:10.3872/j.issn.1007-385X.2023.02.007 Prev Next
Clinical Research
Mechanism of PD-L1 and Siglec-15 in regulating malignant biological behavior of ovarian cancer cells and its clinical significance
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Abstract:
Objective: To investigate the correlation between programmed death-ligand 1 (PD-L1) and sialic acid-binding Ig-like lectin 15 (Siglec-15）in ovarian cancer (OC) and its prognostic significance and their effects on the proliferation, migration and invasion of ovarian cancer SKOV3 cells.Methods: A total of 50 paraffin samples of ovarian cancer tissues and tissues of ipsilateral oviducts resected from patients with OC from January 2017 to December 2019 were collected from the Second Attached Hospital of Fujian Medical University. The expression levels of PD-L1 and Siglec-15 of cancer tissues and oviduct tissues were detected by immunohistochemical staining Envision method. Kaplan-Meier survival and Logistic regression analysis were used to analyze the relationship between the expressions of PD-L1and Siglec-15, and the prognosis of OC patients. Si-PD-L1 and si-NC were respectively transfected in ovarian cancer cells SKOV3 by transient transfection technology. The effect of PD-L1 expression on Siglec-15 in ovarian cancer cells SKOV3 was detected by qPCR and WB assays. CCK-8 and Transwell assays were used to verify the effect of PD-L1 and Siglec-15 expressions on the proliferation, migration and invasion of SKOV3 cells. Results: In the 50 cases of ovarian cancer, PD-L1 and Siglec-15 were highly expressed (50.00% and 42.00%). The expression of PD-L1 is significantly correlated with tumor pathological type, ascites, lymph node metastasis, FIGO stage and recurrence (all P<0.05). The expression of Siglec-15 is significantly correlated with lymph node metastasis and FIGO stage (both P<0.05). Ovarian cancer cell lines with low expression of PD-L1 were successfully constructed.Knockdown of the expression of PD-L1 could increase the expression of Siglec-15. Conclusion: PD-L1 and Siglec-15 have a high positive expression in ovarian cancer tissues. PD-L1 is an independent risk factor for disease recurrence. The expression of PD-L1 is negatively correlated with that of Siglec-15. Knocking down the expression of PD-L1 could increase the expression of Siglec-15, and inhibit the proliferation, migration and invasion of SKOV3 cells.
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