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Archive > Volume 30 Issue 3 > 2023,30(3):223-229. DOI:10.3872/j.issn.1007-385X.2023.03.006 Prev Next
Basic Research
Potential mechanism of Atractylodes macrocephala aqueous extract inhibiting gastric carcinoma through PI3K-Akt-NF-κB signaling pathway
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Abstract:
Objective: To investigate the potential mechanism of Atractylodes macrocephala aqueous extract inhibiting gastric cancer SGC7901 cells. Methods: Gastric perfusion was performed on SD rats using distilled water and Atractylodes macrocephala aqueous extract respectively. Venous blood was collected and insolated to get serum, which was then filtered and named as control group serum (CON-S) and Atractylodes macrocephala group serum (AM-S), respectively. SGC7901 cells were divided into control group, 10% AM-S group and 20% AM-S group. The two AM-S group cells were cultured in AM-S serum of corresponding density for 24 h while the control group cells were cultured with normal medium for the same time. The SGC7901 cells and supernatant were collected for further analysis. MTT assay was used to detect cell viability. Lactate dehydrogenase (LDH), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by commercial kits. Enzyme-linked immuno sorbent assay (ELISA) kits were applied to detect thecontents of interleukin IL-6 and tumor necrosis factor TNF- α in the cells of all the groups. The expressions of phosphatidylinositol 3-kinase PI3K-Akt-NF- κB signaling pathway-related proteins were evaluated by western blot. Results: The proliferative activity of SGC7901 gastric cancer cells in 10% AM-S group and 20% AM-S group decreased by 48.9% and 53.25% respectively compared with the control group (P<0.05 or P<0.01). In the supernatant of gastric cancer cells, compared with the control group, the LDH level of 10% AM-S group and 20% AM-S group increased by 29.25% and 123%; the SOD activity increased by 18% and 54.60%; the MDA leveldecreased by 27.8% and 40.0%; the IL-6 level decreased by 15% and 17.5%, and the TNF-α αlevel decreased by 29.71% and 40.16% respectively (P<0.05 or P<0.01). Compared with the control group, the levels of PI3K-Akt-NF-κB signaling pathway-related protein in AM-S group were significantly reduced (P<0.05 or P<0.01). Conclusion: Atractylodes macrocephala aqueous extract can inhibit gastric cancer by inhibiting the proliferation activity of cancer cells, promoting apoptosis, inhibiting the production of pro-inflammatory factors in tumor microenvironment and changing the level of intracellular oxidative stress, the mechanism of which might be that these anti-cancerous effects are achieved by inhibiting PI3K-Akt-NF-κB signaling pathway.
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