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Archive > Volume 30 Issue 3 > 2023,30(3):237-248. DOI:10.3872/j.issn.1007-385X.2023.03.008 Prev Next
Clinical Research
Clinical significance of ferroptosis-related lncRNAs in prognosis and immune infiltration in clear cell renal cell carcinoma based on bioinformatics analysis
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Abstract:
Objective: To screen lncRNAs related to ferroptosis in renal clear cell carcinoma (ccRCC) by bioinformatics methods, and to explore their correlation with clinical prognosis and immune cell infiltration, in order to provide new targets for the treatment of ccRCC patients. Methods: Transcript data and clinical data of ccRCC were downloaded from The Cancer Genome Atlas (TCGA) database, and ferroptosis-related lncRNAs were obtained using single-sample gene set enrichment analysis (ssGSEA) and correlation analysis. Through univariate and multivariate regression analyses, the characteristics of lncRNAs related to ferroptosis were constructed, and their prognostic value was further analyzed. R software was used to analyze the relationship between the characteristics of ferroptosis-related lncRNAs and tumor immune infiltration and drug sensitivity. A ferroptosis-related ceRNA network was constructed, and the expression of key lncRNAs in Chinese ccRCC tissues and paraneoplastic tissues (samples from 8 cases surgically resected at the Affiliated Hospital of Southwest Medical University between Dec 2019 and Mar 2021) was verified by qPCR. Results: Kaplan-Meier analysis showed that patients with high ferroptosis scores had lower overall survival (OS) than patients with low ferroptosis scores. Univariate and multivariate regression analyses identified 11 ccRCC ferroptosis-related prognostic lncRNAs, and the nomograms for predicting the prognosis of ccRCC patients at 1, 3, and 5 years were constructed. Immune infiltration analysis showed that the characteristics of ferroptosis-related lncRNAs were closely related to ccRCC immune infiltration, among which LINC01871, PRKAR1B-AS1 and CYTOR were the key lncRNAs regulating tumor immune infiltration. Chemotherapy drug sensitivity analysis showed that high-risk patients were more sensitive to methotrexate, paclitaxel, cisplatin, and doxorubicin. Finally, A ceRNA network containing 3 lncRNAs, 15 miRNAs and 15 mRNAs was constructed. qPCR indicated that LINC01871, LINC00472 and CYTOR were significantly up-regulated in ccRCC tissues. Conclusion: Eleven ferroptosis-related lncRNAs were obtained by bioinformatics methods, and they were proved to be related to ccRCC prognosis, immune infiltration and chemotherapeutic drug sensitivity, providing an important reference for exploring ferroptosis-related lncRNA markers in ccRCC.
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