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Archive > Volume 30 Issue 4 > 2023,30(4):324-330. DOI:10.3872/j.issn.1007-385X.2023.04.007 Prev Next
Clinical Research
Expressions of miR-203a and its target genes in hepatocellular carcinoma tissues and their clinical significance
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Abstract:
Objective: To investigate the expressions of miR-203a and its target genes in serum and tumor tissues of patients with hepatocellular carcinoma (HCC) and their relationships with clinicopathological characteristics and prognosis. Methods: The target genes of miR-203a were predicted from TargetScan, miRDB and PicTar websites using bioinformatics methods, which were verified by double luciferase gene report experiment. The samples of cancer tissues and para-cancerous tissues, serum and clinical data of 96 patients with HCC whose tumors were surgically removed at the Second People's Hospital of Jintan District, Changzhou City from January 2018 to June 2019 were collected. The serum of 90 healthy people was collected as controls. The serum miR-203a level and the expressions of miR-203a and its target genes in HCC and para-cancerous tissues were detected by qPCR. The expressions of miR-203a and its target genes in HCC patients with different clinicopathological characteristics were comparatively analyzed. The patients were followed-up for 3 years and overall survival (OS) analysis was performed by Kaplan-Meier method. Results: A total of 10 miR-203a-related target genes were screened from databases, including adenomatous polyposis coli (APC), cyclin dependent kinase 6 (CDK6), transcription factor GATA binding protein 6 (GATA6), homeobox D3 (HOXD3), insulin-like growth factor class 1 receptor (IGF1R), insulin-like growth factor binding protein-5 (IGFBP5), potassium intermediate small conductance calcium activated channel subfamily N, member2 (KCNE2), progestin and adipoQ receptor 3 (PAQR3), Protein arginine methyltransferase 5 (PRMT5) and suppressor of cytokine signaling 3 (SOSC3). The expressions of miR-203a, APC and PAQR3 mRNA in the HCC tissues were significantly lower than those in the para-cancerous tissues (all P<0.01), and the expressions of CDK6, GATA6, HOXD3, IGF1R, IGFBP5, KCNE2、PRMT5 and SOSC3 mRNA were significantly higher than those in the para-cancerous tissues (all P<0.01). The expressions of serum miR-203a and miR-203a and its target genes in HCC tissues were related to the clinical stage, differentiation degree, liver function grade and OS rate of the patients (all P<0.01). Conclusion: miR-203a in HCC tissues is in low expression, and the expressions of miR-203a and target genes are related to clinical stage, differentiation, liver function and long-term OS rate.
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