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Archive > Volume 30 Issue 5 > 2023,30(5):387-392. DOI:10.3872/j.issn.1007-385X.2023.05.004 Prev Next
Basic Research
Effects of deguelin on the proliferation and apoptosis of ovarian cancer SKOV3 cells by regulating miR-520a-3p
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Abstract:
Objective: To investigate the effects of deguelin on the proliferation and apoptosis of ovarian cancer SKOV3 cells by regulating miR-520a-3p. Methods: The SKOV3 cells were divided into the control group (deguelin 0 μmol/L), low-dose deguelin group (5 μmol/L), medium-dose deguelin group (10 μmol/L), and high-dose deguelin group (20 μmol/L), miR-NC group, miR-520a-3p group, deguelin+anti-miR-NC group and deguelin+anti-miR-520a-3p group. CCK-8, colony formation experiment, flow cytometry and qPCR were used to detect the inhibition rate, the number of clone formation, the apoptosis rate and the miR-520a-3p expression of SKOV3 cells respectively. Results: Compared with the control group, the inhibition rate, the apoptosis rate, miR-520a-3p expression of SKOV3 cells in the deguelin (low, medium, and high dose) groups were significantly increased (all P<0.05), and the number of clone formation was significantly reduced (P<0.05). Compared with the miR-NC group, the inhibitory rate and the apoptosis rate of SKOV3 cells in the miR-520a-3p group were significantly increased (all P<0.05), and the number of clone formation was significantly reduced (P<0.05). Compared with the deguelin+anti-miR-NC group, the inhibition rate and the apoptosis rate of SKOV3 cells in the deguelin+anti-miR-520a-3p group were significantly reduced (all P<0.05), and the number of clone formation was significantly increased (P<0.05). Conclusion: Deguelin inhibited the proliferation potential of ovarian cancer SKOV3 cells and induced cell apoptosis by increasing the expression of miR-520a-3p.
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