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Archive > Volume 30 Issue 8 > 2023,30(8):656-664. DOI:10.3872/j.issn.1007-385X.2023.08.002 Prev Next
Basic Research
Construction of a MnO2/Crudlan composite hydrogel and its killing effect on melanoma B16-F10 cells combined with photothermal therapy
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Abstract:
Objective: Crudlan (Cur) was loaded with manganese dioxide (MnO2) nanoparticle to construct a composite hydrogel MnO2@Cur (abbreviated as MGel) and its killing effect on melanoma B16-F10 cells was studied. Methods: Firstly, Cur hydrogel (Gel) was prepared by thermal induction method, and then MnO2 was physically loaded to construct MGel. The macroscopic and microscopic morphology of MGel was characterized and its mechanical properties, degradation properties and photothermal conversion properties were detected. The photothermal killing effect of MGel combined with PTT on mouse cutaneous melanoma B16-F10 cells was studied. Results: MGel is excellent in mechanical strength and degradation performance. Its tensile strength reached (127.97±3.60) kPa，and compressive strength reached (151.44±5.23) kPa; its degradation rate on the 28th day was about 58.17%. MGel obtained excellent photothermal conversion capability after loading MnO2 nanosheets with the average particle diameter of about 180 nm. After receiving 808 nm NIR illumination at 1.0 W/cm2 for 4 minutes, MGel loaded with 1.0 mg/mL MnO2 reached a maximum temperature of 50 ℃. Cytotoxicity experiments and Calcein-AM/PI fluorescence double staining showed that MGel combined with PTT killed B16-F10 melanoma cells effectively, and NIR illumination reduced the cell survival rate of the MGel group to (4.68±0.66)% (P<0.000 1). Conclusion: Composite hydrogel MGel has excellent mechanical strength, degradation performance and photothermal conversion capability. MGel combined with PTT can kill tumor cells effectively, which provides a novel strategy for the treatment of melanoma.
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