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Archive > Volume 30 Issue 8 > 2023,30(8):695-700. DOI:10.3872/j.issn.1007-385X.2023.08.007 Prev Next
Basic Research
Carnosic acid affects the proliferation, migration, and invasion of gastric cancer AGS cells by regulating CXCR7/CXCL12 axis
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Abstract:
Objective: To investigate the effects of carnosic acid (CA) on the proliferation, migration, and invasion of gastric cancer cells by regulating CXC chemokine receptor 7 (CXCR7)/CXC chemokine ligand 12 (CXCL12) axis. Methods: CCK-8 method was used to select appropriate concentrations of CA. Gastric cancer AGS cells were treated with CA at different concentrations (0, 5, 10, 20, 40, 80 μg/mL). AGS cells were divided into the control group (untreated AGS cells), CA group (20 μg/mL CA treatment), CA+ siCXCR7 group (siCXCR7 transfection+20 μg/mL CA treatment), CA+siNC group (siNC transfection+20 μg/mL CA treatment), CA+ vectorNC group (vectorNC transfection+20 μg/mL CA treatment), and CA+vectorCXCR7 group (vectorCXCR7 transfection+20 μg/mL CA treatment). The proliferation of AGS cells was detected by CCK-8 method; the expression levels of CXCR7 and CXCL12 mRNA in cells were detected by qPCR; and the invasion of cells was detected by Transwell assay; the change of cell migration ability was detected by scratch assay, and the expressions of cyclin D1, Bcl-2, CXCR7, CXCL12 and MMP-2 were detected by WB assay. Results: Different concentrations of CA were all able to inhibit the survival rate of AGS cells. When the concentration was 20 μg/mL, the survival rate of AGS cells was closed to 50%. 20 μg/mL CA was therefore chosen for subsequent research. Compared with the control group, the proliferation rate, invasion number, migration rate, cyclin D1, MMP-2, Bcl-2, CXCR7, CXCL12 mRNA and protein expression of the CA group decreased significantly (all P<0.05). Compared with the CA+siNC group, the proliferation rate, invasion number, migration rate, cyclin D1, MMP-2, Bcl-2, CXCR7, CXCL12 mRNA and protein expression in the CA+siCXCR7 group decreased significantly (all P<0.05). Compared with the CA+vectorNC group, the proliferation rate, invasion number, migration rate, cyclin D1, MMP-2, Bcl-2, CXCR7, CXCL12 mRNA and protein expression in the CA+vectorCXCR7 group increased significantly (all P<0.05). Conclusion: CA can inhibit the proliferation, migration, and invasion of AGS cells, which may be related to the inhibition of CXCR7/CXCL12 axis.
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