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Archive > Volume 30 Issue 8 > 2023,30(8):707-714. DOI:10.3872/j.issn.1007-385X.2023.08.009 Prev Next
Clinical Research
circNEIL3 regulates the proliferation, migration and invasion of breast cancer MDA-MB-231 cells through targeting miR-4784
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Abstract:
Objective: To study the influence of circRNA nei-like DNA glucoamylase 3 (circNEIL3) and microRNA (miR)-4784 on the proliferation, migration, and invasion of breast cancer MDA-MB-231 cells. Methods: The cancer tissues and paired paracancerous tissues surgically removed from 45 breast cancer patients histopathologically diagnosed with breast cancer in Jinan Hospital of Integrated Traditional Chinese and Western Medicine from January 2018 to December 2019 were collected. qPCR were applied to measure the relative levels of circNEIL3 and miR-4784 in breast cancer tissues and paracancerous tissues. circNEIL3 small interfering RNA (si-circNEIL3), miR-4784 mimics, and si-circNEIL3+miR-4784 inhibitors were transfected into MDA-MB-231 breast cancer cells, respectively. CCK-8 assay, plate cloning assay, scratch healing assay, and Transwell assay were performed to detect the effects of circNEIL3 and miR-4784 expressions on the cell viability, clone formation, migration and invasion. Dual-luciferase reporter assay, RNA immunoprecipitation (RIP), and RNA pull-down assay were used to detect the interactions between circNEIL3 and miR-4784. Results: The relative level of circNEIL3 in breast cancer tissues was significantly higher than that in adjacent tissues (P<0.05) while the relative level of miR-4784 was significantly lower than that in adjacent tissues (P<0.05). Interference with circNEIL3 significantly reduced the viability, clonal formation numbers, wound healing rate, and invasion numbers of MDA-MB-231 cells (P< 0.05). Overexpression of miR-4784 significantly reduced the viability, clonal formation numbers, wound healing rate, and invasion numbers of MDA-MB-231 cells (P<0.05). Dual luciferase reporter gene assay, RIP, and RNA pull-down assay confirmed that circNEIL3 directly binds to miR-4784. Interference with circNEIL3 significantly up-regulated miR-4784 expression (P<0.05), and overexpression of circNEIL3 significantly down-regulated miR-4784 expression (P<0.05). miR-4784 inhibition partially reversed the inhibitory effect of interference with circNEIL3 on the viability, colony formation, migration, and invasion of MDA-MB-231 cells (P<0.05). Conclusion: Interference with circNEIL3 inhibits the proliferation, migration, and invasion of breast cancer cells by targeting and up-regulating miR-4784 expression.
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