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Archive > Volume 30 Issue 9 > 2023,30(9):771-776. DOI:10.3872/j.issn.1007-385X.2023.09.004 Prev Next
Basic Research
Expression of low-density lipoprotein receptor-associated protein 11 in colorectal cancer tissues and its effect on proliferation and apoptosis of SW480 cells
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Abstract:
Objective: To study the expression and biological function of low density lipoprotein receptor associated protein 11 (LRP11) in colorectal cancer (CRC) tissues and its effect on proliferation and apoptosis of CRC SW480 cells. Methods: The expression of LRP11 in CRC tissues in TCGA database was detected by bioinformatics tool. sh-LRP11 and sh-NC plasmids were transfected into SW480 cells by lentiviral infection technique, respectively. CCK-8 assay was used to detect cell viability, flow cytometry was used to detect cell apoptosis and cell cycle, and Western blot assay was used to detect the expression levels of cyclin D1, BAX, Bcl-2, β-catenin and active- β -catenin in the transfected cells. Results: As shown by TCGA database analysis, compared with normal tissues, the mRNA expression level of LRP11 in CRC tissues was significantly increased (P<0.05). Compared with sh-NC group, the proliferation activity of SW480 cells in sh-LRP11 group was obviously decreased (P<0.05), the apoptosis rate was significantly increased (P<0.01), and the mRNA expression of apoptosis-related protein BAX was increased (P<0.01), while the expression of Bcl-2 was decreased (P<0.01); moreover, the cells in G0/G1 phase was increased significantly and the cells in S-phase was decreased significantly (all P<0.01);meanwhile, the protein expression levels of cyclin D1 and β -catenin and active- β -catenin in wnt/β -catenin signaling pathway were significantly decreased (all P<0.01). Conclusion: The mRNA expression of LRP11 is highly expressed in CRC tissues, and interfering with LRP11 expression can inhibit the proliferation and promote the apoptosis of colon cancer SW480 cells, which provides a new therapeutic target for the treatment of CRC.
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