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Archive > Volume 30 Issue 9 > 2023,30(9):797-803. DOI:10.3872/j.issn.1007-385X.2023.09.008 Prev Next
Basic Research
Butorphanol affects the proliferation, migration and invasion of osteosarcoma MG-63 cells via Hippo/YAP signaling pathway
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Abstract:
Objective: To investigate the impacts of butorphanol (BPH) on the proliferation, migration and invasion of osteosarcoma (OS) cells and the relevant mechanism. Methods: MG-63 cells were grouped into a control group, a YAP inhibitor group (Verteporfin group), and low, medium and high concentration BPH groups. The cytotoxicity and proliferative activity of each group of cells were detected by MTT, the number of cell clones was detected by clonogenic assay, the apoptosis of cells was detected by flow cytometry, the migration ability of the cells was detected by wound-healing test, and the invasion ability of the cells was detected by Transwell assay. The mRNA expression of E-cadherin, N-cadherin and vimentin was detected by qPCR, and the protein expression of YAP and TAZ was detected by WB assay. Besides, the effects of BPH and verteporfin on growth of transplanted tumors were also observed. Results: Compared with the control group, the cell proliferation activity, the number of clones, the rate of wound healing, the number of invaded cells, the mRNA levels of N-cadherin and vimentin, the protein expression of YAP and TAZ, and the tumor volumes were obviously lower (all P<0.05), while the apoptosis rate, mRNA level of E-cadherin and the transplanted tumor inhibition rate were significantly higher (all P<0.05) in the Verteporfin group and the low, medium and high concentration BPH groups.There were no obvious differences in above observation indices between the high concentration BPH and the Verteporfin group (P>0.05). Conclusion: BPH may inhibit the proliferation, migration and invasion of OS cells by inhibiting Hippo/YAP signaling pathway.
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