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Archive > Volume 30 Issue 10 > 2023,30(10):862-867. DOI:10.3872/j.issn.1007-385X.2023.10.002 Prev Next
Basic Research
Expression of phosphoglycerate-mutase 1 in colorectal cancer tissues and its effects on the prognosis and malignant biological behaviors of cancer cells
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Abstract:
Objective：：To investigate the expression of phosphoglycerate mutase 1 (PGAM1) in colorectal cancer (CRC) tissues and its correlation with the prognosis and explore its effects on the proliferation, migration and invasion of CRC cells. Methods: Cancer tissue samples and clinical data of 30 patients who underwent surgery at Tianjin Medical University Cancer Institute and Hospital between March 2003 and November 2008 were collected. Immunohistochemical staining was used to detect the protein expression of PGAM1 in CRC tissues and analyze the relationship between PGAM1 expression and patients’ clinicopathological characteristics. Kaplan‐Meier survival analysis was employed to compare the overall survival (OS) and progress-free survival (PFS) of patients with high and low PGAM1 expression in order to analyze the relationship between PGAM1 expression and the prognosis. HCT-116 and SW480 cells were transfected respectively with si-PGAM1 and si-NC plasmids using RNA interference technology. Western blotting was used to detect PGAM1 protein expression levels in cells transfected with si-PGAM1 and si-NC. Afterwards, CCK-8 and Transwell assays were used to detect the influence of PGAM1 knockdown on the proliferation, migration, and invasion of CRC cells. Results: In 30 CRC tissue samples, PGAM1 positive staining was localized in the cytoplasm of CRC cells, and 33.3% (10 among 30 samples) showed high PGAM1 expression levels. Although the high expression of PGAM1 had no correlation with sex, age, histological type, tumor size,lymph node metastasis, distant metastasis and TNM stage of CRC patients (all P>0.05), the OS and PFS was significantly shorter in patients with PGAM1 high expression than those with low expression. After PGAM1 knockdown the proliferation, migration and invasion abilities of CRC cells were greatly inhibited (all P<0.05). Conclusion: PGAM1 was highly-expressed in CRC tissues. The high expression of PGAM1 was correlated with poor prognosis. Knocking down PGAM1 significantly inhibited the proliferation,migration and invasion abilities of CRC cells. All these findings suggest that PGAM1 might be a promising biomarker for predicting the prognosis of CRC patients.
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