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Archive > Volume 30 Issue 10 > 2023,30(10):887-892. DOI:10.3872/j.issn.1007-385X.2023.10.006 Prev Next
Basic Research
Effects of evodiamine on the proliferation, migration and invasion of neuroblastoma SK-N-SH cells
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Abstract:
Objective: To investigate whether evodiamine (Evo) regulates the proliferation, migration and invasion of neuroblastoma SK-N-SH cells by regulating the expression of lncRNA LINC00858. Methods: Human neuroblastoma SK-N-SH cells were treated with 3, 6 and 12 μmol/L Evo in vitro. si-NC and si-LINC00858 were transfected into SK-N-SH cells by RNA interference technique; pcDNA and pcDNA-LINC00858 were transfected into SK-N-SH cells and treated with 12 μmol/L Evo. The cells were divided into control group, low dose Evo group, medium dose Evo group, high dose Evo group, si-NC group, si-LINC00858 group, Evo+pcDNA group and Evo+pcDNA-LINC00858 group. qPCR was used to detect the expression level of LINC00858 in the cells of each group. Cell proliferation, migration and invasion abilities were detected by MTT and Transwell assay. The expressions of cyclinD1, MMP-2, MMP-9 and p21 proteins were detected by WB assay. Results: Compared with the control group, the expressions of LINC00858 in SK-N-SH cells in Evo low, medium and high dose groups decreased (all P<0.05). The inhibitory rate of cell proliferation increased significantly, and the number of migration and invasion decreased significantly (all P<0.01). The protein expressions of cyclinD1, MMP-2 and MMP-9 decreased, while the protein expression of p21 increased (all P<0.01). Compared with si-NC group, the cell proliferation inhibition rate, the numbers of migration and invasion and the expressions of related proteins in si-LINC00858 group were the same as those in low-dose, medium-dose and high-dose Evo groups. Compared with Evo+pcDNA group, the proliferation inhibition rate of Evo+pcDNA-LINC00858 group decreased significantly, and the numbers of migration and invasion cells increased significantly (all P<0.01).The protein expressions of cyclinD1, MMP-2 and MMP-9 increased, and the protein expression of p21 decreased (all P<0.05). Conclusion: Evo inhibits the proliferation, migration and invasion of neuroblastoma SK-N-SH cells by down-regulating the expression of LINC00858.
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