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Archive > Volume 30 Issue 11 > 2023,30(11):950-956. DOI:10.3872/j.issn.1007-385X.2023.11.003 Prev Next
Basic Research
Characteristic of immune function decline in the process of “Declined of Vital Qi” caused by aging in mice
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Abstract:
Objective: To investigate the characteristics of “Declined of Vital Qi” in the characterization of immune function decline caused by aging based on a mouse model of progressive aging. Methods: C57BL/6 mice of different months (2, 6 and 15 months) were used to detect and compare the specific abundance of T cells, myeloid-derived suppressor cells (MDSC) and their subpopulations in peripheral blood and spleen of mice by flow cytometry. Results: In the peripheral blood, T cell subsets CD3+CD4+CD44-CD62L+ naive CD4+ T cells (2 vs 6 months old, P=0.137; 2 vs 15 months old, P=0.004; 6 vs 15 months old, P=0.105) and CD3+CD8+CD44-CD62L+ naive CD8+ T cells (2 vs 6 months old, P=0.179; 2 vs 15 months old, P=0.001; 6 vs 15 months old, P=0.015) showed a decrease in proportion to aging. However, the proportion of CD3+CD4+CD44+CD62L+ central memory CD8+ T cells increased with aging (2 vs 6 months old, P=0.01; 2 vs 15 months old, P=0.007; 6 vs 15 months old, P=0.164). The results in spleen experiment showed the same trend. At the same time, the proportion of CD8+ T cells increased gradually with aging (2 vs 6 months old, P=0.027; 2 vs 15 months old, P<0.001; 6 vs 15 months old. P<0.001). The subsets of activated CD8+ T cells with phenotype CD8+CD28+ also increased with age (2 vs 6 months old, P=0.863; 2 vs 15 months old, P=0.016; 6 vs 15 months old, P=0.024). Conclusion: In the process of “Declined of Vital Qi” caused by aging, the changes of different immune cell subsets do not all reflect the characteristics of immunosuppression, although the overall immune function decreases. A single phenotype is difficult to reflect the change of overall immune function.
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