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Archive > Volume 30 Issue 11 > 2023,30(11):965-972. DOI:10.3872/j.issn.1007-385X.2023.11.005 Prev Next
Basic Research
Kaempferol promotes cell autophagy and affects cell proliferation of non-small cell lung cancer H1650 cells via regulating Met/PI3K/Akt/mTOR pathway
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Abstract:
Objective: To explore the specific effect and mechanism of kaempferol on inducing autophagy in non-small cell lung cancer (NSCLC) NCI-H1650 cells. Methods: Human NSCLC cell line NCI-H1650 was cultured and treated with kaempferol at different concentrations. The effects of kaempferol on NSCLC cell viability and proliferation were observed by CCK-8, MTT and EdU methods. LC3 adenovirus infection experiment was performed to investigate the effect of kaempferol on NSCLC cell autophagy. Western blot was used to detect the expression of key proteins of cell autophagy and relevant molecules of Met/PI3K/Akt/mTOR signaling pathway. Meanwhile, qPCR was used to detect the mRNA expression of Met in NCI-H1650 cells after kaempferol treatment. The transplanted tumor model of nude mice was established by using luciferase labeled A549-luc cells. The tumor growth was observed by in vivo animal imaging, and the expression of autophagy related key proteins and molecules of Met/PI3K/Akt/mTOR signaling pathway in the xenograft tissues were detected by Western blot. Results: Kaempferol significantly inhibited the proliferation of NCI-H1650 cells (P<0.05). After kaempferol treatment, the number of autophagosomes in NCI-H1650 cells was significantly increased (P<0.05), the expressions of autophagy related key proteins, LC3B and beclin1, were significantly increased (all P<0.05), and the expression of P62 was significantly decreased (P<0.05). Kaempferol significantly inhibited the mRNA and protein expression of Met, and inhibited the protein expression of p-PI3K p85, PI3K p85, p-Akt and p-mTOR (all P<0.05). Kaempferol inhibited the growth of transplanted tumor (P<0.05) and affected autophagy and the expression of Met/PI3K/Akt/mTOR pathway-related proteins in nude mouse tumor models (all P<0.05). Conclusion: Kaempferol induces autophagy in NSCLC NCI-H1650 cells by affecting the Met/PI3K/Akt/mTOR pathway, which in turn inhibites their proliferative capacity.
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