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Archive > Volume 30 Issue 12 > 2023,30(12):1088-1098. DOI:10.3872/j.issn.1007-385X.2023.12.008 Prev Next
Clinical Research
Expression of cuproptosis-related gene COX17 in breast cancer tissues and cells and its association with clinical features and patient prognosis
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Abstract:
Objective: To explore the expression of COX17 in pan-cancerous tissues and its relationship with tumor immune cell infiltration and the prognosis of patients. Methods: The expressions of COX17 in human normal tissues and pan-cancerous tissues and its relationship with the prognosis of patients, the mutation of COX17 gene, the correlation between the expression level of COX17 and tumor immune microenvironment, the correlation between the expression level of COX17 in invasive breast cancer and the clinicopathological characteristics of patients, the genetic mutation and methylation of COX17 gene in breast cancer cells were analyzed, using a variety of public cancer database data. The functional enrichment analysis of the differentially co-expressed genes of COX17 in breast cancer, the construction of the protein interaction network of COX17 and the functional analysis were carried out.Finally, the expression of COX17 protein in breast cancer tissues of Chinese nationals was detected by immunohistochemistry to verify database analysis results. Results: COX17 mRNA is widely distributed in the whole body and highly expressed in most cancer tissues.COX17 protein is highly expressed in breast cancer and other cancer tissues. The expression level of COX17 mRNA significantly affects the prognosis of patients with breast cancer and other cancers. COX17 gene has a high mutation frequency in a variety of cancer tissues, and its main mutation types are missense mutation, amplification and deep deletion. The expression level of COX17 mRNA was correlated with tumor purity and immune cell infiltration in a variety of tumors. The expression level of COX17 was correlated with the clinical stage, the pathological type, lymph node metastasis, the gender and age of patients. Immunohistochemical detection results confirmed that COX17 protein was also highly expressed in the breast cancer tissues of Chinese nationals. The genetic mutation and modification characteristics of COX17 gene in breast cancer were truncation mutation and promoter hypermethylation, respectively,COX17 protein is related to the expressions of ATOX1 and other proteins and forms a complex interaction network. The differentially expressed genes of COX17 in breast cancer mainly involve biological processes such as oxidoreductase activity, protein translation,oxidative phosphorylation and TNF signaling pathway. Conclusion: COX17 is highly expressed in most pan cancers and is related to the immune cell infiltration of a variety of tumors and the prognosis of patients. COX17 is a potential target for clinical treatment of breast cancer.
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