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Oncolytic Newcastle disease virus inhibits IL-6 induced migration and invasion of human glioblastoma U87MG cells
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Abstract:
Objective: To investigate the effect of oncolytic Newcastle disease virus (NDV) on the proliferation, migration and invasion of human glioblastoma U87MG cells induced by IL-6 and its possible mechanism. Methods: U87MG cells were divided into control group, IL-6 group, NDV group and NDV+IL-6 group. The cells in IL-6 group and NDV+IL-6 group were pretreated with 75 ng/mL IL-6 for 1 h, and the other groups were pretreated with DMEM for 1 h; then, the four groups were treated with DMEM, 75 ng/mL IL-6, 1 Hu NDV, 1 Hu NDV+75 ng/mL IL-6 for 24 h, respectively. MTT assay, cell scratch test and Transwell invasion test were used to detect the effects of NDV and IL-6 on the proliferation, migration and invasion of U87MG cells, respectively. The protein expression levels of JAK2,p-JAK2, STAT3, p-STAT3 and MMP2 were detected by WB method. Results: Compared with the control group, the migration rate and number of invasive cells in IL-6 group were significantly increased (P<0.05 or P<0.01). Compared with IL-6 group, the proliferation rate of U87MG cells in NDV+IL-6 group was significantly decreased (P<0.05), and the migration rate and the number of invasive cells were significantly decreased (all P<0.01). WB results showed that compared with the control group, the p-STAT3/STAT3 ratio in IL-6 group was significantly increased (P<0.01), while the p-JAK2/JAK2 ratio, p-STAT3/STAT3 ratio and MMP-2 protein in NDV group were significantly decreased (P<0.05 or P<0.01); compared with IL-6 group, the p-STAT3/STAT3 ratio and the protein expression of MMP-2 in NDV+IL-6 group were significantly decreased (all P<0.05). Conclusion: NDV can inhibit IL-6 induced migration and invasion of human glioblastoma U87MG cells, and its mechanism may be related to its regulation of JAK2/STAT3 signaling pathway.
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