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Archive > Volume 31 Issue 3 > 2024,31(3):240-246. DOI:10.3872/j.issn.1007-385X.2024.03.005 Prev Next
Basic Research
Triptolide inhibits proliferation, invasion and migration of human breast cancer MCF-7 cells by regulating miR-142-3p/HSP70 pathway
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Abstract:
Objective: To investigate the effects of triptolide (TP) on the malignant biological behaviors of human breast cancer MCF-7 cells through the miR-142-3p/HSP70 signaling pathway. Methods: MCF-7 cells were routinely cultured and divided into 6 groups: control group, TP group, miR-142-3p inhibitor group, TP+inhibitor group, miR-142-3p mimics group and TP+mimics group. The corresponding nucleic acids or plasmids were transfected into MCF-7 cells with transfection reagents. The mRNA expression of miR-142-3p and HSP70 in MCF-7 cells was detected by qPCR method. The proliferation, invasion and migration abilities of MCF-7 cells were detected by EdU cell proliferation assay, Transwell chamber assay, and cell scratch assay, respectively. The protein expression of HSP70 in MCF-7 cells was determined by WB assay. Results: TP or miR-142-3p overexpression significantly promoted the expression of miR-142-3p and HSP70 in MCF-7 cells, while knockdown of miR-142-3p significantly inhibited the expression of miR-142-3p and HSP70 in MCF-7 cells. TP could reverse the effects of miR-142-3p knockdown on the expression of miR-142-3p and HSP70 in MCF-7 cells. TP and miR-142-3p overexpression could significantly inhibit the proliferation, migration and invasion of MCF-7 cells (all P<0.05), while knockdown of miR-142-3p could promote the proliferation, migration and invasion of MCF-7 cells (all P<0.05). TP could reverse the effect of miR-142-3p knockdown on the malignant biological behavior of MCF-7 cells (all P<0.05). Conclusions: TP can inhibit the proliferation, invasion, and migration of MCF-7 cells by regulating the miR-142-3p/HSP70 signaling pathway.
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