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Archive > Volume 31 Issue 6 > 2024,31(6):552-557. DOI:10.3872/j.issn.1007-385X.2024.06.002 Prev Next
Basic Research
Activation of DC and promotion of neovascularization by colon cancer membrane biomimetic copper-based metal-organic framework
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Abstract:
Objective: To explore the potential of colon cancer cell membrane-coated copper-based metal-organic frameworks (MOF@CCM) in anti-tumor activities and promotion of tissue healing. Methods: Copper (Ⅱ) 1, 3, 5-benzenetricarboxylate copperbased metal-organic framework (HKUST-1) was constructed, and coated with colon cancer cell CT26 membrane on its outer layer to obtain MOF@CCM, which was physically characterized. The biocompatibility of MOF@CCM was determined by CCK-8 assay, and the effect of MOF@CCM on the proportion of DC2.4 maturation was analyzed by flow cytometry to verify the potential of MOF@CCM in activating immune cells. Angiogenesis experiments was used to verify the potential of MOF@CCM in promoting HUVEC to form blood vessels. Results: MOF@CCM was successfully synthesized. Transmission electron microscopy showed that its shape was approximately circular and had an obvious core-shell structure. The average particle size of MOF@CCM was (150.5±7.89) nm, and the average Zeta potential was -(5.12±1.67) mV. The results of in vitro experiments showed that compared with the control group, MOF@CCM could significantly increase the proportion of DC2.4 maturation ( PP<0.05 or P<0.01), and cell membrane modification had no significant effect on the pro-neovascularization effect of MOF. Conclusion: The synthesized MOF@CCM has good cell compatibility at the dose used and can significantly promote the maturation of DC2.4 cells and HUVEC angiogenesis. It is expected to become a bifunctional treatment platform for anti-colon cancer treatment and tissue repair promotion.
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