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Archive > Volume 31 Issue 6 > 2024,31(6):598-606. DOI:10.3872/j.issn.1007-385X.2024.06.009 Prev Next
Clinical Research
Expression and functional analysis of endocytosis-related gene FCHO2 in breast cancer
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Abstract:
Objective: To explore the expression of endocytosis-related gene FCHO2 in different subtypes of breast cancer, and its relationship with the prognosis and immune cell infiltration of breast cancer patients. Methods: Immunohistochemical staining and bc-GenExMiner v5.0 database were used to analyze the expression of FCHO2 in different subtypes of breast cancer. The relationship between FCHO2 and the prognosis and immune cell infiltration of different subtypes of breast cancer patients was analyzed using GEO and TIMER database. The protein-protein interaction network of FCHO2 and its correlation with the interacting proteins were analyzed using STRING database and GEPIA database, respectively. KEGG and GO analysis of FCHO2-related genes in breast cancer tissues were performed using UALCAN and DAVID databases. Results： Immunohistochemical staining showed that FCHO2 in luminal and HER2+ breast cancer tissues were highly expressed (both P<0.05), and correlated with the expressions of HER2 and Ki67 (P=0.03 or P=0.007). Both the OS and RFS of luminal breast cancer patients with high expression of FCHO2 were significantly decreased (both P<0.05). FCHO2 protein was related to the expressions of many proteins such as EPS15 and constituted the protein-protein interaction network. KEGG and GO analysis indicated that the expression of FCHO2-related genes in breast cancer tissues were mainly related to biological processes such as circadian rhythm and autophagy, and involved FoxO and TGF-β signaling pathways. The expression of FCHO2 was correlated with immune cell infiltration in tissues of different subtypes of breast cancer. Conclusion: FCHO2 is highly expressed in luminal and HER2+ breast cancer tissues, and is correlated with the patient prognosis and immune cell infiltration of luminal breast cancer. FCHO2 may become a potential target for breast cancer treatment.
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