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Archive > Volume 31 Issue 8 > 2024,31(8):786-795. DOI:10.3872/j.issn.1007-385X.2024.08.006 Prev Next
Basic Research
Peroxisomal membrane protein 4 promotes epithelial-mesenchymal transition in hepatocellular carcinoma cells through the ERK1/2 signaling pathway
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Abstract:
Objective: To investigate the impact of peroxisomal membrane protein 4 (PXMP4) on the migration, invasion, and epithelial-mesenchymal transition (EMT) of hepatocellular carcinoma (HCC) cells. Methods: A total of 38 pairs of HCC and paracancerous tissue samples were collected from the Department of Pathology, the Third Affiliated Hospital of Xinxiang Medical College from January 2018 to December 2022. Bioinformatics and immunohistochemistry were used to analyze PXMP4 expression in HCC tissues and its correlation with clinicopathological features of HCC patients. PXMP4 was silenced in HCCLM3 and MHCC97H cells and overexpressed in Huh7 and MHCC97L cells. The silencing/overexpression efficiency was verified by WB assay and qPCR. CCK-8 assay, wound healing assay, and Transwell invasion assay were used to detect the effects of PXMP4 interference or overexpression on the proliferation, migration, and invasion abilities of HCC cells. WB assay was used to detect the protein expression of N-cadherin, E-cadherin, vimentin, extracellular signal-regulated kinase (ERK), and p-ERK in HCC cells with PXMP4 interference/ overexpression or 0, 5, 10, and 20 μmol/L U0126 treatment. Results: Bioinformatics and immunohistochemistry showed that PXMP4 was highly expressed in HCC tissues (PPPPConclusion: PXMP4 is highly expressed in HCC tissues and is closely related to HCC cell differentiation. PXMP4 promotes EMT in HCC cells by activating the ERK1/2 signaling pathway.
Keywords:
hepatocellular carcinoma (HCC) ; peroxisomal membrane protein 4 (PXMP4) ; extracellular signal-regulated kinase (ERK) ; migration ; invasion ; epithelial-mesenchymal transition (EMT)
