Mobile website
Basic Research
Effects of β-1,4-galactosyltransferase on malignant biological behaviors of human breast cancer cells and its possible mechanisms
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
[Abstract] Objective: To investigate the effects of β -1, 4-galactosyltransferase 2 (B4GALT2) on the proliferation, migration and invasion of human breast cancer MCF-7 and MDA-MB-231 cells, and to explore its underlying mechanism. Methods: The mRNA and protein expression of B4GALT2 in breast cancer tissues was analyzed using data from TCGA database and the CPTAC proteomics database. Immunohistochemical staining was used to validate the expression of B4GALT2 protein in breast cancer tissues of Chinese population. The correlation between B4GALT2 mRNA expression in breast cancer tissues and patient prognosis was analyzed using data from the Kaplan-Meier Plotter database. MCF-7 and MDA-MB-231 cells were routinely cultured and transfected with siNC, siRNA#1, siRNA#2, empty vector, and B4GALT2 overexpression plasmids using transfection reagents. The transfected cells were classified as NC group, siRNA#1 group, siRNA#2 group, empty vector group, and OE-B4GALT2 group accordingly. CCK-8 assay and clone formation assay were used to detect the effects of B4GALT2 knockdown on proliferation of transfected cells. Scratch healing assay and Transwell chamber assay were applied to evaluate the effects of B4GALT2 knockdown on cell migration and invasion. WB assay was used to detect the phosphorylation levels of the PI3K/AKT signaling pathway in MCF-7, MDA-MB-231 cells after B4GALT2 knockdown or overexpression. Results: B4GALT2 was highly expressed in breast cancer tissues at both mRNA and protein levels (both P < 0.01), and B4GALT2 protein was also highly expressed in Chinese breast cancer tissues (P < 0.000 1), confirming the initial findings. High B4GALT2 expression was significantly associated with shorter overall prognosis (OS), recurrence-free survival (RFS), and post-progression survival (PPS) (P < 0.01, P < 0.05, P < 0.001) in patients. After B4GALT2 knockdown, the proliferation, migration and invasion of MCF-7 and MDA-MB-231 cells were significantly suppressed (all P < 0.01), and the PI3K/AKT signaling pathway was significantly inhibited (all P < 0.01). The PI3K/AKT signaling pathway was significantly activated after B4GALT2 overexpression (all P < 0.01). Conclusion: B4GALT2 is highly expressed in breast cancer tissues. It promotes the malignant biological behaviors of MCF-7 and MDA-MB-231 cells by regulating the PI3K/AKT signaling pathway.
Keywords:
Project Supported:
Clc Number:
