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Role of METTL3-mediated m6 A modification in regulating miR-1224-5p in the proliferation and migration of prostate cancer cells
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Abstract:
[Abstract] Objective: To investigate the biological role of miR-1224-5p in the proliferation, migration and apoptosis of prostate cancer cells, as well as its regulatory mechanism of expression. Methods: Human prostate cancer cells (PC3, DU145, LNCaP, 22Rv1) and normal prostate epithelial RWPE-1 cells were selected for this study. The expression of miR-1224-5p in prostate cancer cells was detected by qPCR. miR-1224-5p mimic, inhibitor and corresponding negative control (NC) plasmids were transfected into PC3 and DU145 cells by liposome transfection technology, respectively. The transfection efficiency was verified by qPCR. The effects of miR-1224-5p mimic and inhibitor on cell proliferation, migration and apoptosis were detected by CCK-8 assay, plate clone formation assay, scratch healing assay and flow cytometry. The potential N6 -methyladenosine (m6 A) modification sites in the pri-miR-1224-5p sequence were predicted using SRAMP website, and the prediction results were verified using methylated RNA immunoprecipitation (MeRIP). The expression of methyltransferase 3 (METTL3), a key methyltransferase involved in m6 A modification, in prostate cancer cells was detected by qPCR. CCK-8 assay and Transwell assay were used to detect the effect of METTL3 siRNA transfection on the proliferation and migration of PC3 and DU145 cells. The regulatory effect of METTL3-mediated m6 A modification on the expression of miR-1224-5p was detected by qPCR and MeRIP. Results: miR-1224-5p was upregulated in prostate cancer cells (all P < 0.01). Transfection with miR-1224-5p mimic promoted the proliferation, migration and inhibited the apoptosis of PC3 and DU145 cells (P < 0.05 or P < 0.01). Conversely, miR-1224-5p inhibitor suppressed the proliferation, migration and induced apoptosis of PC3 and DU145 cells (P < 0.05 or P < 0.01). pri-miR-1224-5p contained m6 A modification sites. METTL3 was highly expressed in prostate cancer cells (all P < 0.01). Transfection of METTL3 siRNA inhibited the proliferation and migration of PC3 and DU145 cells (all P < 0.01). METTL3-mediated m6 A modification regulated the expression of miR-1224-5p. Conclusion: miR-1224-5p is upregulated in prostate cancer cells through METTL3-mediated m6 A modification. Down-regulation of miR-1224-5p can inhibit the proliferation, migration and induce apoptosis of prostate cancer cells.
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