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Archive > Volume 32 Issue 2 > 2025,32(2):169-175. DOI:10.3872/j.issn.1007-385X.2025.02.005 Prev Next
Basic Research
Effects of targeting chemokine ligand 1 on the malignant biological behaviors of oral squamous cell carcinoma cells
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Abstract:
[Abstract] Objective: To explore the expression of chemokine ligand 1 (CCL1) in oral squamous cell carcinoma (OSCC) and its effect on the proliferation, migration and invasion of human oral tongue squamous cell carcinoma cells (HSC-4). Methods: 28 OSCC tissue samples and clinical characteristic data of patients were collected at the Affiliated Stomatological Hospital of Southwest Medical University between January 2018 and June 2020, as well as 10 normal gingival tissue samples removed during the extraction of impacted teeth. OSCC cells HSC-4 were cultured routinely and divided into the control group (without virus), the NC group (transfected with control lentiviral vector), the shCCL1 group (transfected with knockdown CCL1 lentiviral vector), and the CCL1 group (culture medium containing 60 ng/mL CCL1 recombinant protein). Immunohistochemistry and WB were used to detect the expression of CCL1 in OSCC tissues and cells, and analyze the correlation between its expression level and the clinical features of patients. qPCR, CCK-8 assay, plate cloning assay, cell scratch test, Transwell assay and flow cytometry were used to detect the expression of CCL1 mRNA, the proliferation, migration and invasion abilities and the apoptosis of HSC-4 cells respectively. Results: CCL1 protein was highly expressed in OSCC tissues and HSC-4 cells (all P < 0.01) and its expression was related to the clinical stage of tumors (P < 0.05). The expression of CCL1 in HSC-4 cells was successfully knocked down (P < 0.000 5). Knocking down the expression of CCL1 could inhibit the proliferation (P < 0.05 or P < 0.01), migration and invasion (all P < 0.05) of HSC-4 cells, and promote its apoptosis (all P < 0.05). CCL1 recombinant protein treatment resulted in the opposite effects (P < 0.05, P < 0.01, P < 0.000 1). Conclusion: CCL1 is highly expressed in OSCC and its expression is correlated with the clinical stage of OSCC. CCL1 may take part in regulating the proliferation, migration, invasion and apoptosis of HSC-4 cells.
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