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Volume 6,Issue 1,1999 Table of Contents
1999, 6(1):4-7.
DOI: 10.3872/j.issn.1007-385X.1999.1.002
Abstract:
Objective: Immunotoxin rRTA:DS27, which was prepared by conjugating DS27 with recombinanl ricin a chain, was compared with ricin:DS27 as immunotoxins. Methods: System analysis were performed regard to their cell-specific cytotoxicity, inhibition on the proliferation of hemoapeutic potential cells, immunogold-labelled intracellular routing and effect of NH_(4)Cl on the cytotoxicity. Results: Results showed that rRTA: DS27 got a more specific cytotoxicity and a weaker inhibition on the proliferation of hemoapeutic potential cells than ricin:DS27, NH_(4)Cl could obsolutelyy enhence the cytotoxicity of rRTA:DS27. Conclusion: rRTA:DS27 had more advantages than ricin: DS27 as immunotoxins.
Objective: Immunotoxin rRTA:DS27, which was prepared by conjugating DS27 with recombinanl ricin a chain, was compared with ricin:DS27 as immunotoxins. Methods: System analysis were performed regard to their cell-specific cytotoxicity, inhibition on the proliferation of hemoapeutic potential cells, immunogold-labelled intracellular routing and effect of NH_(4)Cl on the cytotoxicity. Results: Results showed that rRTA: DS27 got a more specific cytotoxicity and a weaker inhibition on the proliferation of hemoapeutic potential cells than ricin:DS27, NH_(4)Cl could obsolutelyy enhence the cytotoxicity of rRTA:DS27. Conclusion: rRTA:DS27 had more advantages than ricin: DS27 as immunotoxins.
1999, 6(1):8-11.
DOI: 10.3872/j.issn.1007-385X.1999.1.003
Abstract:
目的:探索荧光蛋白作为报告基因以及His·Tag作为纯化标签在重组痘苗病毒表达系统中的应用.方法:将N-端融合His·Tag基因序列,插入痘苗病毒表达载体pSFJ2-16,并在其多克隆位点下游装入带有巨细胞病毒(CMV)启动子的突变型荧光蛋白基因(GFP),构建成N-端融合His·Tag并带有荧光蛋白报告基因的分离型痘苗病毒表达载体.再从pET-28中切出C-端融合His·Tag及多克隆位点序列,并与CMV-FP基因一同装入pSFJ2-16,构建成C-端融合His·Tag的分离型痘苗病毒表达载体.将人免疫缺陷病毒-1型(HIV-1)核心蛋白(gag)p17-24基因分别插入上述载体,并筛选出重组病毒.结果:实验表明,重组病毒表达了gag蛋白.结论:将His-Tag的纯化标签加入到通用型痘苗病毒表达载体中用于纯化表达的目的蛋白,该方法是可行的.荧光蛋白基因作为一个筛选标记基因应用于痘苗病毒载体系统还有待进行进一步研究.
目的:探索荧光蛋白作为报告基因以及His·Tag作为纯化标签在重组痘苗病毒表达系统中的应用.方法:将N-端融合His·Tag基因序列,插入痘苗病毒表达载体pSFJ2-16,并在其多克隆位点下游装入带有巨细胞病毒(CMV)启动子的突变型荧光蛋白基因(GFP),构建成N-端融合His·Tag并带有荧光蛋白报告基因的分离型痘苗病毒表达载体.再从pET-28中切出C-端融合His·Tag及多克隆位点序列,并与CMV-FP基因一同装入pSFJ2-16,构建成C-端融合His·Tag的分离型痘苗病毒表达载体.将人免疫缺陷病毒-1型(HIV-1)核心蛋白(gag)p17-24基因分别插入上述载体,并筛选出重组病毒.结果:实验表明,重组病毒表达了gag蛋白.结论:将His-Tag的纯化标签加入到通用型痘苗病毒表达载体中用于纯化表达的目的蛋白,该方法是可行的.荧光蛋白基因作为一个筛选标记基因应用于痘苗病毒载体系统还有待进行进一步研究.
1999, 6(1):12-16.
DOI: 10.3872/j.issn.1007-385X.1999.1.004
Abstract:
Objective: To investigate the effects of DC derived from IL-12-induced erythroleukemia cells on the induction of CTL and protective antituinor immunity. Methods: The cytotoxicity of CTL was assessed by 4h 51 Cr-release assay. HE staining and eleclromicroscopy were used to observe the histological changes after immunized with erythroleukemia-derived DCs. Results: After incubation with naive T cells, the IL-12-induced FBL-3 cells could apparently induce the generation of CTL which exihibit the specific cytotoxicity against wild-type FBL-3 cells in vitro. We further assayed the cytotoxicity of CTL in vivo after immunized with erythroleukemia-derived DCs. CTL cytotoxicity of mice immunized with IL-12-in-duced FBL-3 cells was higher than that of mice immunized with IL-I2-uninduced FBL-3 cells. C57BL/6 mice immunized with IL-12-induced FBL-3 cells could resistant the subsequent rechallenge with the wild-type FBL-3 cells. The tumor growth was efficiently inhibited. Histological observation showed that more inflammatory cells infiltrated into the tumor tissue and necrosis of leukemia cells existed. By transmission electron microscopy, apoptositic phenomena were observed in the tumor of group immunized with IL-12-induced FBL-3 cells. However, these changes were not observed in the group immunized with IL-12-uninduced FBL-3 cells. Conclusion: These data indicate that erythroleukemia cells-derived DCs could induce specific CTL efficiently in vitro and in vivo, and may be used as new vaccine to activate antituinor immune responses.
Objective: To investigate the effects of DC derived from IL-12-induced erythroleukemia cells on the induction of CTL and protective antituinor immunity. Methods: The cytotoxicity of CTL was assessed by 4h 51 Cr-release assay. HE staining and eleclromicroscopy were used to observe the histological changes after immunized with erythroleukemia-derived DCs. Results: After incubation with naive T cells, the IL-12-induced FBL-3 cells could apparently induce the generation of CTL which exihibit the specific cytotoxicity against wild-type FBL-3 cells in vitro. We further assayed the cytotoxicity of CTL in vivo after immunized with erythroleukemia-derived DCs. CTL cytotoxicity of mice immunized with IL-12-in-duced FBL-3 cells was higher than that of mice immunized with IL-I2-uninduced FBL-3 cells. C57BL/6 mice immunized with IL-12-induced FBL-3 cells could resistant the subsequent rechallenge with the wild-type FBL-3 cells. The tumor growth was efficiently inhibited. Histological observation showed that more inflammatory cells infiltrated into the tumor tissue and necrosis of leukemia cells existed. By transmission electron microscopy, apoptositic phenomena were observed in the tumor of group immunized with IL-12-induced FBL-3 cells. However, these changes were not observed in the group immunized with IL-12-uninduced FBL-3 cells. Conclusion: These data indicate that erythroleukemia cells-derived DCs could induce specific CTL efficiently in vitro and in vivo, and may be used as new vaccine to activate antituinor immune responses.
Ren Huan
,
Xing Shuxian
,
Xu Hongwei
,
Song Yinghui
,
Shang Xiaozhou
,
Zhou Guisheng
,
Tian Jingxian
,
Li Dianjun
1999, 6(1):17-21.
DOI: 10.3872/j.issn.1007-385X.1999.1.005
Abstract:
Objective: To investigate the proliferation profile and the cytoloxicity of CIK (cytokine induced killer) cells, in vitro, and their anti-tumor effects on S180 bearing mice in vitro. Methods: CIK cells were generated by culturing PBMC in the presence of r-IFN on day 0 and IL-2, anti-CD3 McAb, IL-1 on day 1; CIK cultures were analyzed on different time points by FACS; Compared with LAK cells, the cytotoxicity in vitro by MTT assays and the anti-tumor effects on S180 bearing mice in vivo of CIK cells were tested respectively. Results: The flow cytometry analysis showed that CD3 CD56 T cells which were the major cytolytic effectors in CIK cells expanded 1 000-fold after 2 weeks in CIK culture ; CIK cells had greater cytotoxicity against tumor cells in vitro than that of LAK cells; and had also significantly anti-tumor effects in mice bearing S180 tumor compared with LAK cells in vivo. (median inhibitory rates 78% vs 33%, P < 0.05 t ); The CIK therapy was possibly correlated with induction of the activation of the splenic T lymphocytes of S180 bearing mice. Conclusion: CIK cells were highly efficient cytolytic effector cells which were non-MHC restricted
Objective: To investigate the proliferation profile and the cytoloxicity of CIK (cytokine induced killer) cells, in vitro, and their anti-tumor effects on S180 bearing mice in vitro. Methods: CIK cells were generated by culturing PBMC in the presence of r-IFN on day 0 and IL-2, anti-CD3 McAb, IL-1 on day 1; CIK cultures were analyzed on different time points by FACS; Compared with LAK cells, the cytotoxicity in vitro by MTT assays and the anti-tumor effects on S180 bearing mice in vivo of CIK cells were tested respectively. Results: The flow cytometry analysis showed that CD3 CD56 T cells which were the major cytolytic effectors in CIK cells expanded 1 000-fold after 2 weeks in CIK culture ; CIK cells had greater cytotoxicity against tumor cells in vitro than that of LAK cells; and had also significantly anti-tumor effects in mice bearing S180 tumor compared with LAK cells in vivo. (median inhibitory rates 78% vs 33%, P < 0.05 t ); The CIK therapy was possibly correlated with induction of the activation of the splenic T lymphocytes of S180 bearing mice. Conclusion: CIK cells were highly efficient cytolytic effector cells which were non-MHC restricted
1999, 6(1):22-26.
DOI: 10.3872/j.issn.1007-385X.1999.1.006
Abstract:
Objective: To amplify the tk gene of HSV- I strain Stocker and clone into a eukaryotic expressing plasmid. A high effective expressing vector containing HSV-I tk gene would be constructed. Methods: PCR amplification was performed using primers based on tk gene sequence of HSV-I strain CL101, nucleotide of strain Stocker as template. PCR product was cloned into plasmid pUCl 19 and the sequence of tk gene was analyzed. The tk gene was then cloned into a high effective eukaryotic expressing plasmid which contains CMV immediate early gene promoter. The recombinant pCR3-tk was further identified by restriction digest. Results: A 1427 bp DNA fragment was amplified. Sequence analysis and restriction digest demonstrated that the fragment cloned contained complete HSV-I tk and indicated a 98.5% identity between the cloned tk gene and that from HSV-I CL101. pCR3-tk vector was identified by restrition digest. Conclusion: The identity between the cloned tk gene and that from HSV-I CL101 is very high. The recombinant of pCR3-tk is a high effective expressing eukaryotic vector. It will play a fundamental role in further study of tumor gene therapy with HSV- I tk/GCV system expecially mediated by non-viral vectors such as cationic liposomes.
Objective: To amplify the tk gene of HSV- I strain Stocker and clone into a eukaryotic expressing plasmid. A high effective expressing vector containing HSV-I tk gene would be constructed. Methods: PCR amplification was performed using primers based on tk gene sequence of HSV-I strain CL101, nucleotide of strain Stocker as template. PCR product was cloned into plasmid pUCl 19 and the sequence of tk gene was analyzed. The tk gene was then cloned into a high effective eukaryotic expressing plasmid which contains CMV immediate early gene promoter. The recombinant pCR3-tk was further identified by restriction digest. Results: A 1427 bp DNA fragment was amplified. Sequence analysis and restriction digest demonstrated that the fragment cloned contained complete HSV-I tk and indicated a 98.5% identity between the cloned tk gene and that from HSV-I CL101. pCR3-tk vector was identified by restrition digest. Conclusion: The identity between the cloned tk gene and that from HSV-I CL101 is very high. The recombinant of pCR3-tk is a high effective expressing eukaryotic vector. It will play a fundamental role in further study of tumor gene therapy with HSV- I tk/GCV system expecially mediated by non-viral vectors such as cationic liposomes.
1999, 6(1):27-30.
DOI: 10.3872/j.issn.1007-385X.1999.1.007
Abstract:
Objective: To investigate the therapeutic effects of the murine IL-12 retrovirus packaging cell line on hep-atoma injected locally. Methods: The retrovirus vector encoding mIL-12 gene was constructed and transfected into packaging cell line PA317. The cells were then used to treat the rats with experimental orthotopic hepatoma at different time. The therapeutic effects, immune functions of the hosts, pathological and toxicological responses were documented. Results: The results showed that the mIL-12 retrovirus packaging cell line could significantly inhibit the growth of the hepatoma cells injected locally to the hepatoma. The early treatment made the rats survive long, while the medium or late stage treatment could prolong the life time of the rats compared with the bland control group or bland vector control group, though the rats did not survive. The number of NK cells and T cells increased significantly in the treatment group. The effects of the early treatment were superior to those of the medium and late stage treatment. Moreover, the transfection of IL-12 gene locally in the hepatoma tissue could make the hepatoma disappear from other liver lobe. This phenomenon demonstrated that IL-12 could activate the immune cells of the host to kill the untransfected tumor cells. This is very important for IL-12 to be used in gene therapy clinically. Meanwhile, the hepatoma would not recur in the rats that had survived more than 2 months from the early treatment after being rechallenged with tumor cells. Conclusion: The results showed that IL-12 gene injected locally in the hepatoma tissue could enhance the anti-tumor immunity of the host.
Objective: To investigate the therapeutic effects of the murine IL-12 retrovirus packaging cell line on hep-atoma injected locally. Methods: The retrovirus vector encoding mIL-12 gene was constructed and transfected into packaging cell line PA317. The cells were then used to treat the rats with experimental orthotopic hepatoma at different time. The therapeutic effects, immune functions of the hosts, pathological and toxicological responses were documented. Results: The results showed that the mIL-12 retrovirus packaging cell line could significantly inhibit the growth of the hepatoma cells injected locally to the hepatoma. The early treatment made the rats survive long, while the medium or late stage treatment could prolong the life time of the rats compared with the bland control group or bland vector control group, though the rats did not survive. The number of NK cells and T cells increased significantly in the treatment group. The effects of the early treatment were superior to those of the medium and late stage treatment. Moreover, the transfection of IL-12 gene locally in the hepatoma tissue could make the hepatoma disappear from other liver lobe. This phenomenon demonstrated that IL-12 could activate the immune cells of the host to kill the untransfected tumor cells. This is very important for IL-12 to be used in gene therapy clinically. Meanwhile, the hepatoma would not recur in the rats that had survived more than 2 months from the early treatment after being rechallenged with tumor cells. Conclusion: The results showed that IL-12 gene injected locally in the hepatoma tissue could enhance the anti-tumor immunity of the host.
Yang Jiahe
,
Qian Qijun
,
Xue Huibin
,
Cao Huifang
,
Cui Zhenfu
,
Shi Wenfang
,
Wei Lixin
,
Yang Guangshun
,
Wu Mengchao
1999, 6(1):31-34.
DOI: 10.3872/j.issn.1007-385X.1999.1.008
Abstract:
Objective: To investigate the antitumor effects and the possible mechanisms of tumor antigen peptide-pulsed, IL-2 gene-modified dendritic cells (DCs) in combination with low-dose cyclophosphamide (Cy). Methods: Mutl is a MHC class I -restricted tumor antigen peptide of 3LL Lewis lung carcinoma. The mice bearing metastatic lung carcinoma were treated by vaccination with DCs transfected with IL-2 gene and pulsed with Mutl, then the survival period of mice were observed. The phenotypes of splenocytes were detected by FACS and the cytotoxicity of CTL and NK were assayed by 4h-51Cr release assay. Results: The combind treatment could inhibit pulmonary metastasis of tumor most significantly and increase the proportion of the CD8 T cells, NK1.1 cells in spleen mononuclear cells. The highest CTL cytotoxicity could be induced. Conclusion: Tumor antigen peptide-pulsed,IL-2 gene-modified DCs combined with low-dose Cy could induce antitumor immune response most potently and then inhibit tumor pulmonary metastasis most significantly.
Objective: To investigate the antitumor effects and the possible mechanisms of tumor antigen peptide-pulsed, IL-2 gene-modified dendritic cells (DCs) in combination with low-dose cyclophosphamide (Cy). Methods: Mutl is a MHC class I -restricted tumor antigen peptide of 3LL Lewis lung carcinoma. The mice bearing metastatic lung carcinoma were treated by vaccination with DCs transfected with IL-2 gene and pulsed with Mutl, then the survival period of mice were observed. The phenotypes of splenocytes were detected by FACS and the cytotoxicity of CTL and NK were assayed by 4h-51Cr release assay. Results: The combind treatment could inhibit pulmonary metastasis of tumor most significantly and increase the proportion of the CD8 T cells, NK1.1 cells in spleen mononuclear cells. The highest CTL cytotoxicity could be induced. Conclusion: Tumor antigen peptide-pulsed,IL-2 gene-modified DCs combined with low-dose Cy could induce antitumor immune response most potently and then inhibit tumor pulmonary metastasis most significantly.
1999, 6(1):35-38.
DOI: 10.3872/j.issn.1007-385X.1999.1.009
Abstract:
Fas； β-雌二醇受体； 融合基因； 表达； 凋亡；
Fas； β-雌二醇受体； 融合基因； 表达； 凋亡；
Wang Jianhua
,
Tong Shanqing
,
Jiang Jiu
,
Ding Jianqing
,
Hu Baoyu
,
Zhu Youming
,
Lu Deyuan
,
Hua Zude
,
Lu Jing
1999, 6(1):39-44.
DOI: 10.3872/j.issn.1007-385X.1999.1.010
Abstract:
目的:研究卵巢癌肿瘤浸润淋巴细胞在体外扩增后某些细胞因子的表达.方法:利用RT-PCR和免疫学方法,分离5例刚分离的上皮性卵巢癌肿瘤浸润淋巴细胞(TIL)和其中3例腹水标本中肿瘤相关淋巴细胞(TAL),检测其在体外扩增后,IL-2,IL-2R,TNF-α,IFN-γ,IL-6等细胞因子基因的表达的水平与变化;测定细胞培养上清液中细胞因子的活性,分析TIL或TAL扩增前后杀伤肿瘤细胞的能力和免疫学特性的变化.结果:TIL在扩增前后的4周时间内,仅TNF-α mRNA基因表达是持续的,并可维持相当长一段时间;IFN-γmRNA基因的表达缺乏;IL-2mRNA基因表达只是一过性.体外加入抗CD3单抗或PHA(合适浓度)刺激TIL或TAL,IL-2,TNF-α,IhN-γmRNA表达明显增强.和TIL细胞因子基因表达不尽相同的是,TAL表达IL-6 mR-NA.结果还发现,体外IL-2的浓度对TIL的免疫学特性有很大的影响.结论:TIL在体外经rIL-2扩增后,免疫活性有明显提高.TIL的抗肿瘤活性在很大程度上依靠其分泌的多种细胞因子.
目的:研究卵巢癌肿瘤浸润淋巴细胞在体外扩增后某些细胞因子的表达.方法:利用RT-PCR和免疫学方法,分离5例刚分离的上皮性卵巢癌肿瘤浸润淋巴细胞(TIL)和其中3例腹水标本中肿瘤相关淋巴细胞(TAL),检测其在体外扩增后,IL-2,IL-2R,TNF-α,IFN-γ,IL-6等细胞因子基因的表达的水平与变化;测定细胞培养上清液中细胞因子的活性,分析TIL或TAL扩增前后杀伤肿瘤细胞的能力和免疫学特性的变化.结果:TIL在扩增前后的4周时间内,仅TNF-α mRNA基因表达是持续的,并可维持相当长一段时间;IFN-γmRNA基因的表达缺乏;IL-2mRNA基因表达只是一过性.体外加入抗CD3单抗或PHA(合适浓度)刺激TIL或TAL,IL-2,TNF-α,IhN-γmRNA表达明显增强.和TIL细胞因子基因表达不尽相同的是,TAL表达IL-6 mR-NA.结果还发现,体外IL-2的浓度对TIL的免疫学特性有很大的影响.结论:TIL在体外经rIL-2扩增后,免疫活性有明显提高.TIL的抗肿瘤活性在很大程度上依靠其分泌的多种细胞因子.
1999, 6(1):45-49.
DOI: 10.3872/j.issn.1007-385X.1999.1.011
Abstract:
Objective: To investigate the antitumor effects and the possible mechanisms of tumor antigen peptide-pulsed, IL-2 gene-modified dendritic cells (DCs) in combination with low-dose cyclophosphamide (Cy). Methods: Mutl is a MHC class I -restricted tumor antigen peptide of 3LL Lewis lung carcinoma. The mice bearing metastatic lung carcinoma were treated by vaccination with DCs transfected with IL-2 gene and pulsed with Mutl, then the survival period of mice were observed. The phenotypes of splenocytes were detected by FACS and the cytotoxicity of CTL and NK were assayed by 4h-51Cr release assay. Results: The combind treatment could inhibit pulmonary metastasis of tumor most significantly and increase the proportion of the CD8 T cells, NK1.1 cells in spleen mononuclear cells. The highest CTL cytotoxicity could be induced. Conclusion: Tumor antigen peptide-pulsed,IL-2 gene-modified DCs combined with low-dose Cy could induce antitumor immune response most potently and then inhibit tumor pulmonary metastasis most significantly.
Objective: To investigate the antitumor effects and the possible mechanisms of tumor antigen peptide-pulsed, IL-2 gene-modified dendritic cells (DCs) in combination with low-dose cyclophosphamide (Cy). Methods: Mutl is a MHC class I -restricted tumor antigen peptide of 3LL Lewis lung carcinoma. The mice bearing metastatic lung carcinoma were treated by vaccination with DCs transfected with IL-2 gene and pulsed with Mutl, then the survival period of mice were observed. The phenotypes of splenocytes were detected by FACS and the cytotoxicity of CTL and NK were assayed by 4h-51Cr release assay. Results: The combind treatment could inhibit pulmonary metastasis of tumor most significantly and increase the proportion of the CD8 T cells, NK1.1 cells in spleen mononuclear cells. The highest CTL cytotoxicity could be induced. Conclusion: Tumor antigen peptide-pulsed,IL-2 gene-modified DCs combined with low-dose Cy could induce antitumor immune response most potently and then inhibit tumor pulmonary metastasis most significantly.
1999, 6(1):50-51.
DOI: 10.3872/j.issn.1007-385X.1999.1.012
Abstract:
Objective: Immunotoxin rRTA:DS27, which was prepared by conjugating DS27 with recombinanl ricin a chain, was compared with ricin:DS27 as immunotoxins. Methods: System analysis were performed regard to their cell-specific cytotoxicity, inhibition on the proliferation of hemoapeutic potential cells, immunogold-labelled intracellular routing and effect of NH_(4)Cl on the cytotoxicity. Results: Results showed that rRTA: DS27 got a more specific cytotoxicity and a weaker inhibition on the proliferation of hemoapeutic potential cells than ricin:DS27, NH_(4)Cl could obsolutelyy enhence the cytotoxicity of rRTA:DS27. Conclusion: rRTA:DS27 had more advantages than ricin: DS27 as immunotoxins.
Objective: Immunotoxin rRTA:DS27, which was prepared by conjugating DS27 with recombinanl ricin a chain, was compared with ricin:DS27 as immunotoxins. Methods: System analysis were performed regard to their cell-specific cytotoxicity, inhibition on the proliferation of hemoapeutic potential cells, immunogold-labelled intracellular routing and effect of NH_(4)Cl on the cytotoxicity. Results: Results showed that rRTA: DS27 got a more specific cytotoxicity and a weaker inhibition on the proliferation of hemoapeutic potential cells than ricin:DS27, NH_(4)Cl could obsolutelyy enhence the cytotoxicity of rRTA:DS27. Conclusion: rRTA:DS27 had more advantages than ricin: DS27 as immunotoxins.
1999, 6(1):52-60.
DOI: 10.3872/j.issn.1007-385X.1999.1.013
Abstract:
Objective: To investigate the proliferation profile and the cytoloxicity of CIK (cytokine induced killer) cells, in vitro, and their anti-tumor effects on S180 bearing mice in vitro. Methods: CIK cells were generated by culturing PBMC in the presence of r-IFN on day 0 and IL-2, anti-CD3 McAb, IL-1 on day 1; CIK cultures were analyzed on different time points by FACS; Compared with LAK cells, the cytotoxicity in vitro by MTT assays and the anti-tumor effects on S180 bearing mice in vivo of CIK cells were tested respectively. Results: The flow cytometry analysis showed that CD3 CD56 T cells which were the major cytolytic effectors in CIK cells expanded 1 000-fold after 2 weeks in CIK culture ; CIK cells had greater cytotoxicity against tumor cells in vitro than that of LAK cells; and had also significantly anti-tumor effects in mice bearing S180 tumor compared with LAK cells in vivo. (median inhibitory rates 78% vs 33%, P < 0.05 t ); The CIK therapy was possibly correlated with induction of the activation of the splenic T lymphocytes of S180 bearing mice. Conclusion: CIK cells were highly efficient cytolytic effector cells which were non-MHC restricted.
Objective: To investigate the proliferation profile and the cytoloxicity of CIK (cytokine induced killer) cells, in vitro, and their anti-tumor effects on S180 bearing mice in vitro. Methods: CIK cells were generated by culturing PBMC in the presence of r-IFN on day 0 and IL-2, anti-CD3 McAb, IL-1 on day 1; CIK cultures were analyzed on different time points by FACS; Compared with LAK cells, the cytotoxicity in vitro by MTT assays and the anti-tumor effects on S180 bearing mice in vivo of CIK cells were tested respectively. Results: The flow cytometry analysis showed that CD3 CD56 T cells which were the major cytolytic effectors in CIK cells expanded 1 000-fold after 2 weeks in CIK culture ; CIK cells had greater cytotoxicity against tumor cells in vitro than that of LAK cells; and had also significantly anti-tumor effects in mice bearing S180 tumor compared with LAK cells in vivo. (median inhibitory rates 78% vs 33%, P < 0.05 t ); The CIK therapy was possibly correlated with induction of the activation of the splenic T lymphocytes of S180 bearing mice. Conclusion: CIK cells were highly efficient cytolytic effector cells which were non-MHC restricted.
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- 《中国肿瘤生物治疗杂志》
- 1994年创刊
- 主办单位：Chinese Society of Immunology, Chinese Anti-cancer Association
- 邮编：200433
- 电话：021-81871002-22
- 电子邮箱：cjcb@biother.cn
- 网址:http://www.biother.cn
- 刊号：ISSN 1007-385X
- CN 31-1725/R
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Supported by:Beijing E-Tiller Technology Development Co., Ltd.