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Volume 8,Issue 4,2001 Table of Contents
2001, 8(4):239-242.
DOI: 10.3872/j.issn.1007-385X.2001.4.001
Abstract:
2001, 8(4):243-247.
DOI: 10.3872/j.issn.1007-385X.2001.4.002
Abstract:
研究腺病毒介导的KDR启动子-胸苷激酶系统对血管内皮细胞的选择性杀伤作用.方法应用PCR克隆出人KDR基因启动子序列-225 bp～+127 bp,以AdEasy system为载体,构建携带受KDR启动子或CMV启动子调控tk基因表达的2种重组腺病毒质粒pAdKDR-tk和pAdCMV-tk,在293细胞中包装、扩增后,体外感染表达KDR的脐静脉血管内皮细胞系HUVEC和不表达KDR的肝癌细胞系HepG2,并给予不同浓度的丙氧鸟苷(GCV)处理,5 d后收集存活细胞并计数.结果产生的病毒滴度均为5×109pfu/ml.在MOI为100、GCV为50μg/ml条件下,AdKDR-tk转染HUVEC后细胞生存率下降(31.49%±6.42%),AdKDR-tk转染HepG2后细胞生存率为76.57%±3.49%,而转染AdCMV-tk的2细胞系生存率均显著下降,细胞生存率分别为22.24%±3.77%(HUVEC)和26.53%±6.84%(HepG2).结论KDR基因启动子可调控HSV-tk在血管内皮细胞中特异性表达,为进一步开展靶向肿瘤血管内皮的自杀基因治疗研究奠定了基础.
研究腺病毒介导的KDR启动子-胸苷激酶系统对血管内皮细胞的选择性杀伤作用.方法应用PCR克隆出人KDR基因启动子序列-225 bp～+127 bp,以AdEasy system为载体,构建携带受KDR启动子或CMV启动子调控tk基因表达的2种重组腺病毒质粒pAdKDR-tk和pAdCMV-tk,在293细胞中包装、扩增后,体外感染表达KDR的脐静脉血管内皮细胞系HUVEC和不表达KDR的肝癌细胞系HepG2,并给予不同浓度的丙氧鸟苷(GCV)处理,5 d后收集存活细胞并计数.结果产生的病毒滴度均为5×109pfu/ml.在MOI为100、GCV为50μg/ml条件下,AdKDR-tk转染HUVEC后细胞生存率下降(31.49%±6.42%),AdKDR-tk转染HepG2后细胞生存率为76.57%±3.49%,而转染AdCMV-tk的2细胞系生存率均显著下降,细胞生存率分别为22.24%±3.77%(HUVEC)和26.53%±6.84%(HepG2).结论KDR基因启动子可调控HSV-tk在血管内皮细胞中特异性表达,为进一步开展靶向肿瘤血管内皮的自杀基因治疗研究奠定了基础.
2001, 8(4):248-252.
DOI: 10.3872/j.issn.1007-385X.2001.4.003
Abstract:
To explore the optimal combination of HGF for expanding CD34 cells and the suitable time for transplantation.Methods: CB CD34 cells were cultured for 22 days in medium containing following combination of HGFs: Group A (Containing no HGFs as control), group B (SCF,IL-3,IL-6), group C (SCF,IL-3,IL-6,G-CSF,Epo),group D (SCF,IL-3,IL-6,TPO,Flt3-L), group E (SCF,IL-3,IL-6,TPO,Flt3-L,G-CSF,Epo). At every intervals, the cells were collected and assayed for CD. Results: NC and CD34 cells were expanded significantly in all expansion group, the peak of number of NC is on day 10 and that of CD34 cells is on day 6. The highest level of NC is in group E, and that of CD34 and CD34 CD38 - cells is in group D. The content of CD34 and CD34 CD38 - cells decreased gradually. Interestingly, the content of CD34 CD38 - cells in group B and D increased slightly on day 6 and that of CD14 ,CD13 ,CD61 Gly-A cells elevated increasingly correspondingly. However, the content of CD3 and CD19 cells decreased during expansion. Conclusions: Differentiation of CD34 cells related to combination of HGF and time of ex vivo expansion , SCF combines with IL-3,IL-6,TPO,Flt3-L,G-CSF and Epo is optimal combination regarding to the number of NC, but in view of the content of HSPC, the combination containing SCF,IL-3,IL-6,TPO and Flt3-L is suitable for expansion of HSPC. The content of HSPC reached the highest level in first week, so the suitable time for transplantation of expanded cells is from day 6 to day 10 of expansion.
To explore the optimal combination of HGF for expanding CD34 cells and the suitable time for transplantation.Methods: CB CD34 cells were cultured for 22 days in medium containing following combination of HGFs: Group A (Containing no HGFs as control), group B (SCF,IL-3,IL-6), group C (SCF,IL-3,IL-6,G-CSF,Epo),group D (SCF,IL-3,IL-6,TPO,Flt3-L), group E (SCF,IL-3,IL-6,TPO,Flt3-L,G-CSF,Epo). At every intervals, the cells were collected and assayed for CD. Results: NC and CD34 cells were expanded significantly in all expansion group, the peak of number of NC is on day 10 and that of CD34 cells is on day 6. The highest level of NC is in group E, and that of CD34 and CD34 CD38 - cells is in group D. The content of CD34 and CD34 CD38 - cells decreased gradually. Interestingly, the content of CD34 CD38 - cells in group B and D increased slightly on day 6 and that of CD14 ,CD13 ,CD61 Gly-A cells elevated increasingly correspondingly. However, the content of CD3 and CD19 cells decreased during expansion. Conclusions: Differentiation of CD34 cells related to combination of HGF and time of ex vivo expansion , SCF combines with IL-3,IL-6,TPO,Flt3-L,G-CSF and Epo is optimal combination regarding to the number of NC, but in view of the content of HSPC, the combination containing SCF,IL-3,IL-6,TPO and Flt3-L is suitable for expansion of HSPC. The content of HSPC reached the highest level in first week, so the suitable time for transplantation of expanded cells is from day 6 to day 10 of expansion.
2001, 8(4):253-256.
DOI: 10.3872/j.issn.1007-385X.2001.4.004
Abstract:
观察u-PAR反义核酸载体对高侵袭为PC－3M亚系侵袭能力的抑制效应。方法：利用单层细胞侵袭和软琼脂克5隆形成实验，对比观察u-PAR反义核酸对高侵袭亚系体外侵袭能力的影响；应用RT－PCR和明前酶谱法，分析u-PAR反义核酸对高侵袭亚系MMP－9活性的影响，并且观察u-PAR反义核酸载体转染前后的细胞亚系在裸鼠体内成瘤率及转移情况。结果：转染u-PAR反义核酸的高侵袭亚系体外侵袭能力和非贴壁依赖性生长能力均明显降低，抑制率分别为79％和60％，u-PAR反义核酸虽不影响高侵袭亚系MMP－9 mRNA的转录，但对MMP－9酶原的激活有抑制作用；且转染u-PAR反义核酸载体亚系的体内成瘤率和移植瘤的生长明显受到抑制，与对照组之间差异具有极显著性（P＜0．01）。结论：u-PAR反义核酸对高侵袭亚系体外侵袭能力有较强抑制作用，并可显著抑制高侵袭亚系的体内生长能力。
观察u-PAR反义核酸载体对高侵袭为PC－3M亚系侵袭能力的抑制效应。方法：利用单层细胞侵袭和软琼脂克5隆形成实验，对比观察u-PAR反义核酸对高侵袭亚系体外侵袭能力的影响；应用RT－PCR和明前酶谱法，分析u-PAR反义核酸对高侵袭亚系MMP－9活性的影响，并且观察u-PAR反义核酸载体转染前后的细胞亚系在裸鼠体内成瘤率及转移情况。结果：转染u-PAR反义核酸的高侵袭亚系体外侵袭能力和非贴壁依赖性生长能力均明显降低，抑制率分别为79％和60％，u-PAR反义核酸虽不影响高侵袭亚系MMP－9 mRNA的转录，但对MMP－9酶原的激活有抑制作用；且转染u-PAR反义核酸载体亚系的体内成瘤率和移植瘤的生长明显受到抑制，与对照组之间差异具有极显著性（P＜0．01）。结论：u-PAR反义核酸对高侵袭亚系体外侵袭能力有较强抑制作用，并可显著抑制高侵袭亚系的体内生长能力。
5 Treatment of the Colonal Cancer with Intratumoral Injection Combining 131 I- CL3 with 131 I-ch TNT
LUO Rong-cheng
,
LI Ai-min
,
LIAO Wang-jun
,
ZHANG Jun-yi
,
DING Xue-mei
,
ZHENG Da-yong
,
ZHENG Hang
2001, 8(4):257-259.
DOI: 10.3872/j.issn.1007-385X.2001.4.005
Abstract:
To define the feasibility and superiority of radioimmunotherapy with l31 I labeled anti-nucleus antigen monoclonal antibody chTNT ( 131 I-chTNT) and l31 I labeled anti-cell membrane antigen CL 3 monoclonal antibody( 131 I-CL 3 )intratumora1 injection, provide experimental evidence for the application of radioimmunotheray with 131 I labeled kinds of monoclonal antibodies. Methods: Nude mice bearing subcutaneous human colonal cancer xenografts were treated by intratumoral injection of l31 I-CL 3 and/or with l31 I-chTNT. Alteration of the tumor size and measured the radioactivity concentration in tumor,liver,blood and kidney were observed.Results: The inhibitory rate(82.3%) in group treated with 131 I-CL 3 and 131 I-chTNT was higher than that of using l31 I-CL 3 (57.9%)or l31 I-chTNT(53 8%) alone.The same was the radioactivity concentration ratios(T/NT) of tumor to normal tissue in three groups. Conclusion: Intratumoral injection with 131 I labeled kinds of monoclonal antibodies can increase the concentration of l31 I labeled monoclonal antibodies in tumor and enhance the efficacy of the radioimmunotherapy.
To define the feasibility and superiority of radioimmunotherapy with l31 I labeled anti-nucleus antigen monoclonal antibody chTNT ( 131 I-chTNT) and l31 I labeled anti-cell membrane antigen CL 3 monoclonal antibody( 131 I-CL 3 )intratumora1 injection, provide experimental evidence for the application of radioimmunotheray with 131 I labeled kinds of monoclonal antibodies. Methods: Nude mice bearing subcutaneous human colonal cancer xenografts were treated by intratumoral injection of l31 I-CL 3 and/or with l31 I-chTNT. Alteration of the tumor size and measured the radioactivity concentration in tumor,liver,blood and kidney were observed.Results: The inhibitory rate(82.3%) in group treated with 131 I-CL 3 and 131 I-chTNT was higher than that of using l31 I-CL 3 (57.9%)or l31 I-chTNT(53 8%) alone.The same was the radioactivity concentration ratios(T/NT) of tumor to normal tissue in three groups. Conclusion: Intratumoral injection with 131 I labeled kinds of monoclonal antibodies can increase the concentration of l31 I labeled monoclonal antibodies in tumor and enhance the efficacy of the radioimmunotherapy.
2001, 8(4):264-268.
DOI: 10.3872/j.issn.1007-385X.2001.4.007
Abstract:
To investigate the effect of anti-HPV16 E6-ribozyme on sensitivity of cervical carcinoma cells line to cisplatin. Methods: With the method of lipofectin transfection, the anti-HPV16E6-ribozyme and the eucaryotic expressing plasmids per se were transfected into CaSKi cells, which were named CaSKi-R, CaSKi-P respectively. The expression of ribozyme in transfected cells was observed by RNA dot blot. The amounts of E6 mRNA in the three kinds of cells were detected by Northern blot. The growth curve and colony formation test of three cell types were detected. Their sensitivity to cisplatin were examined by MTT assay. The apoptosis rates of each cell and expressions of P53, Bcl-2 and Bax was determined by PI/Annexin V stained methods and flow cytometry analysis.Results: Anti-HPV16E6-ribozyme can be expressed stably in transfected CaSKi cells. Northern blot showed that E6 mRNA was less in CaSKi-R than in CaSKi. The growth rate and cloning efficiency of CaSKi-R cells were lower than that of CaSKi and CaSKi-P cells. The sensitivity and apoptotic rates of CaSKi-R cells to cisplatin were higher than that of CaSKi and CaSKi-P cells( P <0.01).The expression of P53, Bax protein was upregulated and the expression of Bcl-2 protein downregulated compared to that of CaSKi and CaSKi-P cells( P <0.01).Conclusion: Anti-HPVE6-ribozyme inhibited CaSKi-R cell growth, cloning efficiency and increased the sensitivity to cisplatin.
To investigate the effect of anti-HPV16 E6-ribozyme on sensitivity of cervical carcinoma cells line to cisplatin. Methods: With the method of lipofectin transfection, the anti-HPV16E6-ribozyme and the eucaryotic expressing plasmids per se were transfected into CaSKi cells, which were named CaSKi-R, CaSKi-P respectively. The expression of ribozyme in transfected cells was observed by RNA dot blot. The amounts of E6 mRNA in the three kinds of cells were detected by Northern blot. The growth curve and colony formation test of three cell types were detected. Their sensitivity to cisplatin were examined by MTT assay. The apoptosis rates of each cell and expressions of P53, Bcl-2 and Bax was determined by PI/Annexin V stained methods and flow cytometry analysis.Results: Anti-HPV16E6-ribozyme can be expressed stably in transfected CaSKi cells. Northern blot showed that E6 mRNA was less in CaSKi-R than in CaSKi. The growth rate and cloning efficiency of CaSKi-R cells were lower than that of CaSKi and CaSKi-P cells. The sensitivity and apoptotic rates of CaSKi-R cells to cisplatin were higher than that of CaSKi and CaSKi-P cells( P <0.01).The expression of P53, Bax protein was upregulated and the expression of Bcl-2 protein downregulated compared to that of CaSKi and CaSKi-P cells( P <0.01).Conclusion: Anti-HPVE6-ribozyme inhibited CaSKi-R cell growth, cloning efficiency and increased the sensitivity to cisplatin.
2001, 8(4):269-273.
DOI: 10.3872/j.issn.1007-385X.2001.4.008
Abstract:
探讨白血病细胞疫苗主动免疫治疗在血液恶性肿瘤中的作用。方法：建立红白血病荷瘤小鼠，用自制的3种不同的白血病细胞瘤苗进行主动免疫治疗，用MTT比色法测其治疗2周和4周的Mφ和CTL的杀伤活性，并与对照组相比较。结果：（1）随着肿瘤细胞在小鼠体内的增长，小鼠的细胞免疫功能受到严重抑制，特异性细胞免疫功能的抑制滞后于非特异性细胞免疫功能。（2）灭活肿瘤细胞＋IFA＋细胞因子（rGM-CSF rIL-2 rIL-6)的肿瘤疫苗在提高荷瘤小鼠的细胞免疫功能方面，尤其是特异性细胞免疫功能方面优于灭活肿瘤细胞＋IFA肿瘤疫苗，而仅含灭活肿瘤细胞的疫苗作用不明显，结论：灭活肿瘤细胞＋IFA＋细胞因子（rGM-CSF rIL-2 rIL-6)的肿瘤疫苗在血液恶性肿瘤的特异性主动免疫治疗中很有潜力。
探讨白血病细胞疫苗主动免疫治疗在血液恶性肿瘤中的作用。方法：建立红白血病荷瘤小鼠，用自制的3种不同的白血病细胞瘤苗进行主动免疫治疗，用MTT比色法测其治疗2周和4周的Mφ和CTL的杀伤活性，并与对照组相比较。结果：（1）随着肿瘤细胞在小鼠体内的增长，小鼠的细胞免疫功能受到严重抑制，特异性细胞免疫功能的抑制滞后于非特异性细胞免疫功能。（2）灭活肿瘤细胞＋IFA＋细胞因子（rGM-CSF rIL-2 rIL-6)的肿瘤疫苗在提高荷瘤小鼠的细胞免疫功能方面，尤其是特异性细胞免疫功能方面优于灭活肿瘤细胞＋IFA肿瘤疫苗，而仅含灭活肿瘤细胞的疫苗作用不明显，结论：灭活肿瘤细胞＋IFA＋细胞因子（rGM-CSF rIL-2 rIL-6)的肿瘤疫苗在血液恶性肿瘤的特异性主动免疫治疗中很有潜力。
2001, 8(4):281-284.
DOI: 10.3872/j.issn.1007-385X.2001.4.011
Abstract:
To observe the curative effect of tumor antigen pulsed dendritic cells (DCs) combined with interleukin 2 (IL-2) activated lymphocytes infused into thoracic cavity and abdominal cavity on the inhibition of pleural fluid and ascites of advanced carcinoma patients, and to investigate the correlated immune mechanism. Methods: The peripheral blood mononuclear cells of patients suffered from advanced carcinoma were separated as a source of monocyte derived dendritic cells (MoDC) and IL-2-activated lymphocytes. MoDC were pulsed with tumor lysates derived from tumor cells of thoracic or abdominal fluid of patients, combined with IL-2-activated lymphocytes and infused into thoracic cavity and abdominal cavity of the patients. The changes of pleural fluid or ascites of patients were examined by X ray or B supersonic wave. The cytokine receptor on lymphocytes was analysis by FACS. Results: After Immunotherapy, the cancer cells in thoracic cavity and abdominal cavity of the patients were inhibited significantly. The expression of IL-2R on lymphocytes in pleural fluid and ascites was up-regulated and IL-10R was down-regulated. The complete remission rate was 46.9%, part remission rate was 51.3%, and effectual rate was 100 0% of patients received Immunotherapy. Curative effect was effective than that of DDP control( P <0.05). Conclusions: The DC pulsed with tumor antigen combined with IL-2-activated lymphocytes infused into thoracic cavity and abdominal cavity can effectively inhibit the growth of tumor cells. The mechanisms involved may be include: the first, DCs pulsed with tumor antigen may be effectively present the antigen to the T cells in pleural fluid and ascites, which can enhance the specific antitumor immunity; the second, IL-2 activated T cells may also be an effective effector to the tumor cells.
To observe the curative effect of tumor antigen pulsed dendritic cells (DCs) combined with interleukin 2 (IL-2) activated lymphocytes infused into thoracic cavity and abdominal cavity on the inhibition of pleural fluid and ascites of advanced carcinoma patients, and to investigate the correlated immune mechanism. Methods: The peripheral blood mononuclear cells of patients suffered from advanced carcinoma were separated as a source of monocyte derived dendritic cells (MoDC) and IL-2-activated lymphocytes. MoDC were pulsed with tumor lysates derived from tumor cells of thoracic or abdominal fluid of patients, combined with IL-2-activated lymphocytes and infused into thoracic cavity and abdominal cavity of the patients. The changes of pleural fluid or ascites of patients were examined by X ray or B supersonic wave. The cytokine receptor on lymphocytes was analysis by FACS. Results: After Immunotherapy, the cancer cells in thoracic cavity and abdominal cavity of the patients were inhibited significantly. The expression of IL-2R on lymphocytes in pleural fluid and ascites was up-regulated and IL-10R was down-regulated. The complete remission rate was 46.9%, part remission rate was 51.3%, and effectual rate was 100 0% of patients received Immunotherapy. Curative effect was effective than that of DDP control( P <0.05). Conclusions: The DC pulsed with tumor antigen combined with IL-2-activated lymphocytes infused into thoracic cavity and abdominal cavity can effectively inhibit the growth of tumor cells. The mechanisms involved may be include: the first, DCs pulsed with tumor antigen may be effectively present the antigen to the T cells in pleural fluid and ascites, which can enhance the specific antitumor immunity; the second, IL-2 activated T cells may also be an effective effector to the tumor cells.
9 The Effects of Adenovirus-Mediated Human cox-2 Antisense RNA on Growth of Esophgeal Carcinoma Cells
2001, 8(4):285-289.
DOI: 10.3872/j.issn.1007-385X.2001.4.012
Abstract:
To construct the recombinant adenovirus encoding human cox-2 antisense RNA and to investigate its effects on proliferation of tumor cells. Methods: The shuttle plasmid encoding antisense cox-2 was constructed by cloning cox-2 cDNA fragment in the reverse direction into the pHCMVSP1A. Then the plasmid pJM17 and the shuttle plasmid were cotransferred into 293 cells with lipofectamine for homologous recombinantion to acquire recombinant adenovirus which was confirmed by PCR . We evaluated how alterations of cox-2 expression in EC9706 cells transfected with adenovirus-mediated human cox-2 antisense RNA expression (Ad-AShcox-2) or adenovirus recombinants carrying LacZ gene (Ad-LacZ), and its effects on cell proliferation were determined by cell growth rate, 3 H-TdR incorporation and immunohistochemistry. Results: The recombinant adenovirus encoding antisense cox-2 fragment Ad-AShcox-2 was obtained with the titer of 0.86 10 12 PFU/ml. Ad-AShcox-2 could reduce the expression of cox-2 , and strongly inhibit cell growth rate and cause cellular death. Simultaneously, 3 H-TdR incorporation was lower than that of control group ( P <0.001) Conclusion: Reduction of cox-2 expression could inhibit esophageal cancer proliferation through inhibiting the synthesis of DNA.
To construct the recombinant adenovirus encoding human cox-2 antisense RNA and to investigate its effects on proliferation of tumor cells. Methods: The shuttle plasmid encoding antisense cox-2 was constructed by cloning cox-2 cDNA fragment in the reverse direction into the pHCMVSP1A. Then the plasmid pJM17 and the shuttle plasmid were cotransferred into 293 cells with lipofectamine for homologous recombinantion to acquire recombinant adenovirus which was confirmed by PCR . We evaluated how alterations of cox-2 expression in EC9706 cells transfected with adenovirus-mediated human cox-2 antisense RNA expression (Ad-AShcox-2) or adenovirus recombinants carrying LacZ gene (Ad-LacZ), and its effects on cell proliferation were determined by cell growth rate, 3 H-TdR incorporation and immunohistochemistry. Results: The recombinant adenovirus encoding antisense cox-2 fragment Ad-AShcox-2 was obtained with the titer of 0.86 10 12 PFU/ml. Ad-AShcox-2 could reduce the expression of cox-2 , and strongly inhibit cell growth rate and cause cellular death. Simultaneously, 3 H-TdR incorporation was lower than that of control group ( P <0.001) Conclusion: Reduction of cox-2 expression could inhibit esophageal cancer proliferation through inhibiting the synthesis of DNA.
LI Gui-fang
,
LUO Yi-zhou
,
DUAN Chi-jun
,
ZHAI Qun
,
SUN Hai
,
LIAO Hui
,
Wen Hong-sheng
,
WANG Xue-zhi
,
GAN Ge
2001, 8(4):290-293.
DOI: 10.3872/j.issn.1007-385X.2001.4.013
Abstract:
To examine and analyze the nm23, p16 gene expression in gastric cancer tissure,and follow up patients 5 years to discuss the relationship between nm23 and p16 gene synergy expression and gastric cancer biological characteristic and prognosis.Methods: nm23 and p16 protein in gastric cancer tissue and control were detected by immunohistochemistry,and the patients had been followed up for 5 years. Results: In 84 samples of gastric cancer, nm23 positive rate was 46.43%, p16 was 44.05%, the positive rate of gastric cancer tissue and metastasitic lymph node was lower than that of normal control, normal tissue near cancer or benign polyp,and these two genes were related to the depth of tumor invasion and clinical stage.The mortality and recurrence-metastasis rate was higher in these low expression group, and had a shorter median survive period. Conclusion: Abnormal expression of nm23 and p16 gene plays a important role in gastric cancer recurrence and devolepment and may be one of markers for evaluating tumor biological behavior and prognosis.
To examine and analyze the nm23, p16 gene expression in gastric cancer tissure,and follow up patients 5 years to discuss the relationship between nm23 and p16 gene synergy expression and gastric cancer biological characteristic and prognosis.Methods: nm23 and p16 protein in gastric cancer tissue and control were detected by immunohistochemistry,and the patients had been followed up for 5 years. Results: In 84 samples of gastric cancer, nm23 positive rate was 46.43%, p16 was 44.05%, the positive rate of gastric cancer tissue and metastasitic lymph node was lower than that of normal control, normal tissue near cancer or benign polyp,and these two genes were related to the depth of tumor invasion and clinical stage.The mortality and recurrence-metastasis rate was higher in these low expression group, and had a shorter median survive period. Conclusion: Abnormal expression of nm23 and p16 gene plays a important role in gastric cancer recurrence and devolepment and may be one of markers for evaluating tumor biological behavior and prognosis.
2001, 8(4):294-296.
DOI: 10.3872/j.issn.1007-385X.2001.4.014
Abstract:
To establish a sensitive and effective method for bioassay of stem cell factor (SCF) in vitro used for evaluation of biological potency of this products. Methods: Leukemia cell line UT-7 was used in the bioassay of rhSCF. The optimal test condition was determined, by comparing different results of MTT staining from different cell concentration and cell culture time. The potency of SCF was calibrated by the National Reference. Results: According to dose-response curve of SCF on UT-7 cell proliferation, the optimal reaction time and dose range were determined. Conclusion: The established method of using UT-7 dependent cell line to test the SCF bioactivity can be used in routine quality control of the biological potency of recombinant SCF.
To establish a sensitive and effective method for bioassay of stem cell factor (SCF) in vitro used for evaluation of biological potency of this products. Methods: Leukemia cell line UT-7 was used in the bioassay of rhSCF. The optimal test condition was determined, by comparing different results of MTT staining from different cell concentration and cell culture time. The potency of SCF was calibrated by the National Reference. Results: According to dose-response curve of SCF on UT-7 cell proliferation, the optimal reaction time and dose range were determined. Conclusion: The established method of using UT-7 dependent cell line to test the SCF bioactivity can be used in routine quality control of the biological potency of recombinant SCF.
2001, 8(4):301-303.
DOI: 10.3872/j.issn.1007-385X.2001.4.017
Abstract:
TNF诱导细胞凋亡的作用是通过许多信号分子共同参与完成的一个网络工程 ,其中TRAFs家族蛋白 ,尤其是TRAF2 ,是这一信号调节网络的中心环节。本文介绍了TRAF2在TNF诱导细胞凋亡过程中的作用及其信号转导途径。TN FR在与TNF结合后发生聚集 ,并募集TRAF2直接与TNFR2胞内段结合 ,或通过结合其他接头分子与TNFR1间接相连 ,TRAF2主要通过激活NIK和JNK/SAPK 2个独立的信号转导途径对细胞发挥凋亡调节作用 ,具有凋亡保护功能。深入研究TRAF2的结构与功能 ,有利于加深对凋亡调节机理的认识。
TNF诱导细胞凋亡的作用是通过许多信号分子共同参与完成的一个网络工程 ,其中TRAFs家族蛋白 ,尤其是TRAF2 ,是这一信号调节网络的中心环节。本文介绍了TRAF2在TNF诱导细胞凋亡过程中的作用及其信号转导途径。TN FR在与TNF结合后发生聚集 ,并募集TRAF2直接与TNFR2胞内段结合 ,或通过结合其他接头分子与TNFR1间接相连 ,TRAF2主要通过激活NIK和JNK/SAPK 2个独立的信号转导途径对细胞发挥凋亡调节作用 ,具有凋亡保护功能。深入研究TRAF2的结构与功能 ,有利于加深对凋亡调节机理的认识。
2001, 8(4):304-305.
DOI: 10.3872/j.issn.1007-385X.2001.4.018
Abstract:
端粒酶是维持染色体末端端粒长度和功能稳定的重要物质，在端粒酶的3个亚单位中，其催化亚单位hTERT与端粒酶的活性，肿瘤的发生，发展，诊断以及恶性程度的判断有着最大的相关关系。对hTERT表达的抑制可能会成为治疗肿瘤的一个极有前途的手段。
端粒酶是维持染色体末端端粒长度和功能稳定的重要物质，在端粒酶的3个亚单位中，其催化亚单位hTERT与端粒酶的活性，肿瘤的发生，发展，诊断以及恶性程度的判断有着最大的相关关系。对hTERT表达的抑制可能会成为治疗肿瘤的一个极有前途的手段。
2001, 8(4):306-308.
DOI: 10.3872/j.issn.1007-385X.2001.4.019
Abstract:
基因变异是生物适应生存环境和进化的重要手段。乙型肝炎病毒基因变异改变了其致病性及对组织的亲嗜性，导致持续感染，逃避疫苗诱导或自然产生免疫，产生抗药性等，给乙肝治疗带来了新的问题，乙型肝炎病毒是引起肝细胞癌的主要原因之一，肝细胞癌中的乙型肝炎病毒变异可能在该病的发病机理中起重要作用。
基因变异是生物适应生存环境和进化的重要手段。乙型肝炎病毒基因变异改变了其致病性及对组织的亲嗜性，导致持续感染，逃避疫苗诱导或自然产生免疫，产生抗药性等，给乙肝治疗带来了新的问题，乙型肝炎病毒是引起肝细胞癌的主要原因之一，肝细胞癌中的乙型肝炎病毒变异可能在该病的发病机理中起重要作用。
2001, 8(4):309-311.
DOI: 10.3872/j.issn.1007-385X.2001.4.020
Abstract:
目前腺病毒载体已成为重要的基因治疗载体，随着分子生物学技术的迅速发展，构建重组腺病毒的方法有了很大改进。本文主要介绍3种快速构建腺病毒载体的新方法：一是细菌内同源重组法构建重组腺病毒；二是细胞外连接法构建重组腺病毒，三是在黏粒中构建重组腺病毒。3种构建腺病毒载体的新方法相比于传统方法，具有简便，快速，实验周期短的优点。
目前腺病毒载体已成为重要的基因治疗载体，随着分子生物学技术的迅速发展，构建重组腺病毒的方法有了很大改进。本文主要介绍3种快速构建腺病毒载体的新方法：一是细菌内同源重组法构建重组腺病毒；二是细胞外连接法构建重组腺病毒，三是在黏粒中构建重组腺病毒。3种构建腺病毒载体的新方法相比于传统方法，具有简便，快速，实验周期短的优点。
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- 《中国肿瘤生物治疗杂志》
- 1994年创刊
- 主办单位：Chinese Society of Immunology, Chinese Anti-cancer Association
- 邮编：200433
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- 刊号：ISSN 1007-385X
- CN 31-1725/R
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Chinese Journal Of Cancer Biotheray ® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Chinese Journal Of Cancer Biotheray ® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.