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Volume 10,Issue 3,2003 Table of Contents
LI Jun-min
,
SHEN Yang
,
CHEN Fang-yuan
,
XIE Yi
,
WANG Chun
,
HOU Jian
,
HONG Xiao-nan
,
WU De-pei
,
CHEN Jia
,
HOU Ming
,
XU Jian-min
,
SHEN Zhi-xiang
2003, 10(3):157-160.
DOI: 10.3872/j.issn.1007-385X.2003.3.001
Abstract:
Objective: To evaluate the efficacy of rituximab combined with cyclophosphamide, vincristine, doxorubicin and prednisone (CHOP regimen) in treating the initially diagnosed diffuse large B cell lymphoma (DLBL). Methods: 2002-04-2003-02, 52 patients were enrolled in this study. Chemotherapy was conducted with cyclophosphamide 600 mg·m-2, vincristine 1.4 mg·m-2, docorubicin 25 mg·m-2 on d1 and prednisone 60 mg·d-1 for successive 5 d, every 3 weeks, for 6 courses. Rituximab was given at the dose of 375 mg·m-2 every 1 or 3 weeks, for 4 or 6 courses. Results: In 50 patients who were evaluated for efficacy, complete response rate and overall response rate were achieved at 60% and 100%, respectively. 50 patients were followed-up for 2～30 weeks (mean 8±5) and estimated progress free survival (PFS) rate of 16 weeks was 87%. The regimen could be well tolerated, and the major adverse reactions were infusion-related response (32%) and hematological toxicities (20%).Conclusion: Rituximab in combined with CHOP can be successfully applied to the therapy of initially diagnosed diffuse large B cell lymphoma, with adverse reactions.
Objective: To evaluate the efficacy of rituximab combined with cyclophosphamide, vincristine, doxorubicin and prednisone (CHOP regimen) in treating the initially diagnosed diffuse large B cell lymphoma (DLBL). Methods: 2002-04-2003-02, 52 patients were enrolled in this study. Chemotherapy was conducted with cyclophosphamide 600 mg·m-2, vincristine 1.4 mg·m-2, docorubicin 25 mg·m-2 on d1 and prednisone 60 mg·d-1 for successive 5 d, every 3 weeks, for 6 courses. Rituximab was given at the dose of 375 mg·m-2 every 1 or 3 weeks, for 4 or 6 courses. Results: In 50 patients who were evaluated for efficacy, complete response rate and overall response rate were achieved at 60% and 100%, respectively. 50 patients were followed-up for 2～30 weeks (mean 8±5) and estimated progress free survival (PFS) rate of 16 weeks was 87%. The regimen could be well tolerated, and the major adverse reactions were infusion-related response (32%) and hematological toxicities (20%).Conclusion: Rituximab in combined with CHOP can be successfully applied to the therapy of initially diagnosed diffuse large B cell lymphoma, with adverse reactions.
ZHANG Li-hong
,
JIA Lin-tao
,
YU Cui-juan
,
BAO Wei
,
JIN Ming
,
ZHAO jing
,
WANG Cheng-ji
,
YANG An-gang
2003, 10(3):161-165.
DOI: 10.3872/j.issn.1007-385X.2003.3.002
Abstract:
Objiective：To investigate the targeted killing effect to ErbB2 antigen positive cells due to the expression of a secreted fusion protein consisting of anti-erB2 single chain antibody and reversed caspase-3 protein. Methods：pCMV-e23scFv-PeⅡ-revcasp-3 was constructed by sub cloning reversed caspase-3 gene to downstream of anti-erbB2 antibody and PE40 domain Ⅱ genes in recombinant pCMV vector and transfect Jurkat cells. The cell lines secreted expressing fusion protein stable were selected. Fusion protein in mediate was detected by ELISA. Then such mediate was used to culture Hela, SKBr3 and SKOV3 cells respectively and their survivals were compared through MTT. Finally Jurkat- pCMV-e23scFv-PEⅡ-revcasp-3 cells were administrated to BALB/c nude mice bearing SKBr3 tumor through their tail veins. Results： Fusion protein could be expressed by Jurkat cells stably and kill SKBr3 and SKOV3 cells which express ErbB2 antigen but had no influence on Hela which does not expressed ErbB2 antigen. Administrating Jurkat- pCMV-e23scFv-PEⅡ-revcasp-3 cells could inhibit SKBr3 tumor in vivo. Conclusions: Secreted expression of the fusion protein consisting of anti-erbB2 antibody and reversed caspase-3 could targetedly induce ErbB2 antigen positive cells to death.
Objiective：To investigate the targeted killing effect to ErbB2 antigen positive cells due to the expression of a secreted fusion protein consisting of anti-erB2 single chain antibody and reversed caspase-3 protein. Methods：pCMV-e23scFv-PeⅡ-revcasp-3 was constructed by sub cloning reversed caspase-3 gene to downstream of anti-erbB2 antibody and PE40 domain Ⅱ genes in recombinant pCMV vector and transfect Jurkat cells. The cell lines secreted expressing fusion protein stable were selected. Fusion protein in mediate was detected by ELISA. Then such mediate was used to culture Hela, SKBr3 and SKOV3 cells respectively and their survivals were compared through MTT. Finally Jurkat- pCMV-e23scFv-PEⅡ-revcasp-3 cells were administrated to BALB/c nude mice bearing SKBr3 tumor through their tail veins. Results： Fusion protein could be expressed by Jurkat cells stably and kill SKBr3 and SKOV3 cells which express ErbB2 antigen but had no influence on Hela which does not expressed ErbB2 antigen. Administrating Jurkat- pCMV-e23scFv-PEⅡ-revcasp-3 cells could inhibit SKBr3 tumor in vivo. Conclusions: Secreted expression of the fusion protein consisting of anti-erbB2 antibody and reversed caspase-3 could targetedly induce ErbB2 antigen positive cells to death.
FAN Dong-mei
,
LIU Yin-xing
,
XIONG Dong-sheng
,
LAI Zeng-zu
,
XU Yuan-fu
,
SHAO Xiao-feng
,
YANG Ming
,
YANG Chun-zheng
2003, 10(3):166-169.
DOI: 10.3872/j.issn.1007-385X.2003.3.003
Abstract:
Objective:To study anti-tumor mechanism of chimeric anti-CD20 antibody fragment Fab′ in vitro. Methods：The effect of anti-CD20 antibody fragment Fab′ on Raji cells growth was observed using MTT; Annexin V-FITC and PI were used to assay Raji cells apoptosis induced by Fab′; bcl-2 gene expression level change in Raji cells induced by Fab′ was assayed using RT-PCR and Western blotting. Results：The result of MTT indicated that the growth of Raji cells was inhibited by chimeric anti-CD20 antibody fragment Fab′ (IC50, 24.2 μg/ml); bcl-2 gene expression level in Raji cells was decreased prominently by Fab′. Conclusions： It is relative between growth inhibition of Raji cells and apoptosis induced by chimeric anti-CD20 antibody fragment Fab′, and bcl-2 level decline was involved.
Objective:To study anti-tumor mechanism of chimeric anti-CD20 antibody fragment Fab′ in vitro. Methods：The effect of anti-CD20 antibody fragment Fab′ on Raji cells growth was observed using MTT; Annexin V-FITC and PI were used to assay Raji cells apoptosis induced by Fab′; bcl-2 gene expression level change in Raji cells induced by Fab′ was assayed using RT-PCR and Western blotting. Results：The result of MTT indicated that the growth of Raji cells was inhibited by chimeric anti-CD20 antibody fragment Fab′ (IC50, 24.2 μg/ml); bcl-2 gene expression level in Raji cells was decreased prominently by Fab′. Conclusions： It is relative between growth inhibition of Raji cells and apoptosis induced by chimeric anti-CD20 antibody fragment Fab′, and bcl-2 level decline was involved.
2003, 10(3):170-174.
DOI: 10.3872/j.issn.1007-385X.2003.3.004
Abstract:
Objective:To investigate the effects of dendritic cells(DCs) loaded with tumor antigens on the proliferation and antitumor activity of tumor-infiltrating lymphocytes(TILs) from hepatocellular carcinoma (HCC) in vitro. Methods:DCs derived from the peripheral blood of patients with HCC were loaded with autologous tumor lysate(Tuly) to generate DC-Tuly and then the DC-Tuly was co-cultured with autologous TILs in low concentration of IL-2. TILs were counted, and the phenotypes of DCs and TILs were assayed by FCM and the cytotoxicity of TILs was determined with LDH method. The concentration of cytokines in TILs culture supernatant such as IFN-γ and TNF-α was detected by ELISA . Results:The expression levels of CD1a, CD80, CD83,CD86, CD40, HLA-DR molecules on the DC-Tuly are notably increased（P<005）. The proliferation of TILs which were induced by DC-Tuly was markedly enhanced (P<0.01) and the cytotoxicity of the TILs against autologous tumor cells was remarkably increased (P<0.01). After co-cultured with DC-Tuly, CD3+TILs were increased（P<0.05）and the percentage of CD4+ TILs was enhanced(P<0.01), however, the percentage of CD8+ TILs was still higher than that of CD4+ TILs. Both concentrations of IFN-γ and TNF-α in the culture supernatant of the TILs distinctly increased on the 1st and 2nd days of co-culturing (P<0.01). Conclusion: DCs loaded with tumor antigens can obviously promote the proliferation and the specific antitumor activity of TILs.
Objective:To investigate the effects of dendritic cells(DCs) loaded with tumor antigens on the proliferation and antitumor activity of tumor-infiltrating lymphocytes(TILs) from hepatocellular carcinoma (HCC) in vitro. Methods:DCs derived from the peripheral blood of patients with HCC were loaded with autologous tumor lysate(Tuly) to generate DC-Tuly and then the DC-Tuly was co-cultured with autologous TILs in low concentration of IL-2. TILs were counted, and the phenotypes of DCs and TILs were assayed by FCM and the cytotoxicity of TILs was determined with LDH method. The concentration of cytokines in TILs culture supernatant such as IFN-γ and TNF-α was detected by ELISA . Results:The expression levels of CD1a, CD80, CD83,CD86, CD40, HLA-DR molecules on the DC-Tuly are notably increased（P<005）. The proliferation of TILs which were induced by DC-Tuly was markedly enhanced (P<0.01) and the cytotoxicity of the TILs against autologous tumor cells was remarkably increased (P<0.01). After co-cultured with DC-Tuly, CD3+TILs were increased（P<0.05）and the percentage of CD4+ TILs was enhanced(P<0.01), however, the percentage of CD8+ TILs was still higher than that of CD4+ TILs. Both concentrations of IFN-γ and TNF-α in the culture supernatant of the TILs distinctly increased on the 1st and 2nd days of co-culturing (P<0.01). Conclusion: DCs loaded with tumor antigens can obviously promote the proliferation and the specific antitumor activity of TILs.
2003, 10(3):175-179.
DOI: 10.3872/j.issn.1007-385X.2003.3.005
Abstract:
Objective：To study the biological characteristics of dendritic cells (DC) derived from peripheral blood of patients with lung cancer.Methods：Peripheral blood mononuclear cells (PBMC) were isolated from 11 lung cancer patients and healthy donors respectively, cultured with GM-CSF (100 μg/L) and IL-4 (50 μg/L). After pulsed with apoptotic lung cancer cells for 24 h, TNF-α（10 μg/L）added or agonist CD40mAb (5 mg/L) for a further induction of 3～4 days. Phenotypes of DC were detected by FCM. The capacities for DC to uptake antigens were monitored at day 7 and day 11 detected by FITC-dextran uptake test.3H-thymidine incorporation test was used to measure the proliferative capability of T cells. Results： Both DC derived from PBMC of lung cancer patients and from PBMC of healthy donors showed high expressions of CD1α, CD80, CD83, CD86 and HLA-DR, which were known as associated markers of mature DC. The 7th day DC derived from lung cancer patients PBMC showed a strong capacity to uptake antigens. DC were induced by TNF-α or CD40mAb, then became mature. DC entirely lost their capacities of uptake antigens, while DC could stimulate autologous and allogenic T cells' proliferation. When matured DC co-incubated with autologous T cells for 48 h, activated T cells could be observed and the number of activated T cells was significantly increased (P<0.05).Conclusion：The functional and mature DC could be induced from peripheral blood of lung cancer patients in vitro.
Objective：To study the biological characteristics of dendritic cells (DC) derived from peripheral blood of patients with lung cancer.Methods：Peripheral blood mononuclear cells (PBMC) were isolated from 11 lung cancer patients and healthy donors respectively, cultured with GM-CSF (100 μg/L) and IL-4 (50 μg/L). After pulsed with apoptotic lung cancer cells for 24 h, TNF-α（10 μg/L）added or agonist CD40mAb (5 mg/L) for a further induction of 3～4 days. Phenotypes of DC were detected by FCM. The capacities for DC to uptake antigens were monitored at day 7 and day 11 detected by FITC-dextran uptake test.3H-thymidine incorporation test was used to measure the proliferative capability of T cells. Results： Both DC derived from PBMC of lung cancer patients and from PBMC of healthy donors showed high expressions of CD1α, CD80, CD83, CD86 and HLA-DR, which were known as associated markers of mature DC. The 7th day DC derived from lung cancer patients PBMC showed a strong capacity to uptake antigens. DC were induced by TNF-α or CD40mAb, then became mature. DC entirely lost their capacities of uptake antigens, while DC could stimulate autologous and allogenic T cells' proliferation. When matured DC co-incubated with autologous T cells for 48 h, activated T cells could be observed and the number of activated T cells was significantly increased (P<0.05).Conclusion：The functional and mature DC could be induced from peripheral blood of lung cancer patients in vitro.
2003, 10(3):180-184.
DOI: 10.3872/j.issn.1007-385X.2003.3.006
Abstract:
Objective: To investigate the effects of Notch1 gene transfection on the growth of human hepatocarcinoma cells and to explore the mechanisms of Notch signaling. Methods: Recombinant retrovirus MSCV-ICN/GFP and MSCV-GFP were prepared by calcium phosphate precipitation mediated gene transduction of 293T package cells. The recombinant retroviruses were used to infect human hepatocarcinoma cells Hep3B. After infected, proliferation of Hep3B cells was observed by MTT assay. Cell cycle distribution was analyzed by FACS and p53 expression was detected by Western blot. Results: After infection of MSCV-ICN/GFP recombinant retrovirus, the growth of Hep3B cells was inhibited significantly and cell cycle distribution analysis showed that Hep3B cells in G0/G1 phase were increased. Western immunoblotting showed that Notch1 signaling up regulated p53 expression. Conclusion: Notch1 activation could inhibit the growth of hepatocarcinoma cells through G0/G1 cell cycle arrest and p53 induction.
Objective: To investigate the effects of Notch1 gene transfection on the growth of human hepatocarcinoma cells and to explore the mechanisms of Notch signaling. Methods: Recombinant retrovirus MSCV-ICN/GFP and MSCV-GFP were prepared by calcium phosphate precipitation mediated gene transduction of 293T package cells. The recombinant retroviruses were used to infect human hepatocarcinoma cells Hep3B. After infected, proliferation of Hep3B cells was observed by MTT assay. Cell cycle distribution was analyzed by FACS and p53 expression was detected by Western blot. Results: After infection of MSCV-ICN/GFP recombinant retrovirus, the growth of Hep3B cells was inhibited significantly and cell cycle distribution analysis showed that Hep3B cells in G0/G1 phase were increased. Western immunoblotting showed that Notch1 signaling up regulated p53 expression. Conclusion: Notch1 activation could inhibit the growth of hepatocarcinoma cells through G0/G1 cell cycle arrest and p53 induction.
PENG Shu-ping
,
FANG Wei-yi
,
DAI Wen-jian
,
ZOU Xiao-qing
,
LIU Hong-ying
,
SHI Shu-hong
,
CAO Jian-guo
2003, 10(3):185-189.
DOI: 10.3872/j.issn.1007-385X.2003.3.007
Abstract:
Objective:To construct cloning and prokaryotic expression vector of vascular basement membrane-derived multifunctional peptide and to analyze its space conformation. Methods:Vascular basement membrane-derived multifunctional peptide (VBMDMP) sequence obtained by polymerase chain reaction was cloned into vector pUC19. We also subcloned it into prokaryotic expression vector pGEX-4T-1. The recombinant was confirmed by restriction endonuclease digestion and auto-sequencer. pGEX-4T-1-VBMDMP was transformed into E. coli JM109 0.1 mmol/L IPTG can induce high expression of GST-VBMDMP. GST-VBMDMP was obtained by Glutathione Sepharose 4B columns.The space conformation of VBMDMP was predicted by using computer program Antheprot. Results: The recombinant VBMDMP gene confirmed by restriction endonuclease digestion and auto-sequencer was consistent with sequence we designed. The high expression of GST-VBMDMP was induced by 0.1 mmol/L IPTG in E.coli JM109. GST-VBMDMP was highly purified. The analysis of software Antheprot demonstrated that the two domains of tumstatin linked by upper hinge region of IgG3 can extend itself freely. Conclusion: The cloning and prokaryotic expression vector was successfully constructed.
Objective:To construct cloning and prokaryotic expression vector of vascular basement membrane-derived multifunctional peptide and to analyze its space conformation. Methods:Vascular basement membrane-derived multifunctional peptide (VBMDMP) sequence obtained by polymerase chain reaction was cloned into vector pUC19. We also subcloned it into prokaryotic expression vector pGEX-4T-1. The recombinant was confirmed by restriction endonuclease digestion and auto-sequencer. pGEX-4T-1-VBMDMP was transformed into E. coli JM109 0.1 mmol/L IPTG can induce high expression of GST-VBMDMP. GST-VBMDMP was obtained by Glutathione Sepharose 4B columns.The space conformation of VBMDMP was predicted by using computer program Antheprot. Results: The recombinant VBMDMP gene confirmed by restriction endonuclease digestion and auto-sequencer was consistent with sequence we designed. The high expression of GST-VBMDMP was induced by 0.1 mmol/L IPTG in E.coli JM109. GST-VBMDMP was highly purified. The analysis of software Antheprot demonstrated that the two domains of tumstatin linked by upper hinge region of IgG3 can extend itself freely. Conclusion: The cloning and prokaryotic expression vector was successfully constructed.
2003, 10(3):190-193.
DOI: 10.3872/j.issn.1007-385X.2003.3.008
Abstract:
Objective: To investigate the therapeutic effects of ets-1 antisense oligoxydeonucleotide(AsODN) on gastric carcinoma. Methods: Cultured SGC-7901 cells were devided into control group, sense oligoxydeonucleotide(sODN) group and AsODN group. After transfection for 24 h, expression of ets-1mRNA was detected by RT-PCR, growth inhibition was detected by colone formation assay, in vitro invasive ability assay was carried out in Transwell chamber，the animal model of xenotransplanted gastric carcinoma in nude mice was established to detect invasive ability in vivo. Results: ets-1 AsODN could under-regulate the expression of ets-1 mRNA, number of colone formation of AsODN group was significantly lower than the other two groups(24.2±4.8 vs 47.6±8.1 vs 44.3±7.6，P<0.01）, so was the number of cells which crossed to the lower surface of the matrigel-coated filters (97.1±11.1 vs 198.7±18.3, 205.8±22.2， P<001）. ets-1 AsODN could also inhibit the growth of xenotransplanted gastric carcinoma in nude mice significantly. Conclusion: ets-1 AsODN had therapeutic effects on gastric cancinoma, and the inhibition of the invasive ability of gastric cancer cells might be one of its mechanisms.
Objective: To investigate the therapeutic effects of ets-1 antisense oligoxydeonucleotide(AsODN) on gastric carcinoma. Methods: Cultured SGC-7901 cells were devided into control group, sense oligoxydeonucleotide(sODN) group and AsODN group. After transfection for 24 h, expression of ets-1mRNA was detected by RT-PCR, growth inhibition was detected by colone formation assay, in vitro invasive ability assay was carried out in Transwell chamber，the animal model of xenotransplanted gastric carcinoma in nude mice was established to detect invasive ability in vivo. Results: ets-1 AsODN could under-regulate the expression of ets-1 mRNA, number of colone formation of AsODN group was significantly lower than the other two groups(24.2±4.8 vs 47.6±8.1 vs 44.3±7.6，P<0.01）, so was the number of cells which crossed to the lower surface of the matrigel-coated filters (97.1±11.1 vs 198.7±18.3, 205.8±22.2， P<001）. ets-1 AsODN could also inhibit the growth of xenotransplanted gastric carcinoma in nude mice significantly. Conclusion: ets-1 AsODN had therapeutic effects on gastric cancinoma, and the inhibition of the invasive ability of gastric cancer cells might be one of its mechanisms.
2003, 10(3):194-197.
DOI: 10.3872/j.issn.1007-385X.2003.3.009
Abstract:
Objective:To analyze the circulating endothelial cells (CECs) and circulating endothelial precursors (CEPs) in peripheral blood of tumor patients. Methods:CECs and CEPs were enumerated in 57 tumor patients and 15 healthy controls by 3-color flow cytometry. The serum level of angiogenesis related cytokines（VEGF, bFGF）was determined by ELISA method.Results：In tumor patients, mean value of CECs and CEPs was (0.378±0.047)% and (0059±0.013)% respectively. The serum level of VEGF and bFGF were (295.58±59.56) ng/L and (28.73±740) ng/L. The amount of CECs/CEPs positively correlated with the serum level of VEGF and bFGF. Conclusions: The CECs/CEPs and serum level of VEGF and bFGF of tumor patients was higher than those of normal controls. VEGF and bFGF may participate into the course of endothelial progenitors mobilization.
Objective:To analyze the circulating endothelial cells (CECs) and circulating endothelial precursors (CEPs) in peripheral blood of tumor patients. Methods:CECs and CEPs were enumerated in 57 tumor patients and 15 healthy controls by 3-color flow cytometry. The serum level of angiogenesis related cytokines（VEGF, bFGF）was determined by ELISA method.Results：In tumor patients, mean value of CECs and CEPs was (0.378±0.047)% and (0059±0.013)% respectively. The serum level of VEGF and bFGF were (295.58±59.56) ng/L and (28.73±740) ng/L. The amount of CECs/CEPs positively correlated with the serum level of VEGF and bFGF. Conclusions: The CECs/CEPs and serum level of VEGF and bFGF of tumor patients was higher than those of normal controls. VEGF and bFGF may participate into the course of endothelial progenitors mobilization.
2003, 10(3):198-201.
DOI: 10.3872/j.issn.1007-385X.2003.3.010
Abstract:
Objective: To investigate the apoptotic effects and its mechanisms on K562 cells caused by Interferon-alpha (INF-α) and Cytarabine (Ara-C). Methods: The variation of both morphology and inhibitory rate of K562 cells was observed in culture medium with IFN-α and various concentrations of Ara-C at different time in vitro. The variation of telomerase activity and P53 protein expression were detected before and after apoptosis occurred. Results:INF-α and Ara-C used concurrently could cause apoptosis and inhibit the growth of K562 cells as well as decrease the telomerase activity and increase P53 protein expression significantly. All these processes showed both in time- and dose-dependent manner. Conclusions: INF-α and Ara-C used concurrently can inhibit the growth of K562 cells and induce apoptosis, inhibiting the telomerase activity and increasing the expression of P53 protein may be one of the most important mechanisms.
Objective: To investigate the apoptotic effects and its mechanisms on K562 cells caused by Interferon-alpha (INF-α) and Cytarabine (Ara-C). Methods: The variation of both morphology and inhibitory rate of K562 cells was observed in culture medium with IFN-α and various concentrations of Ara-C at different time in vitro. The variation of telomerase activity and P53 protein expression were detected before and after apoptosis occurred. Results:INF-α and Ara-C used concurrently could cause apoptosis and inhibit the growth of K562 cells as well as decrease the telomerase activity and increase P53 protein expression significantly. All these processes showed both in time- and dose-dependent manner. Conclusions: INF-α and Ara-C used concurrently can inhibit the growth of K562 cells and induce apoptosis, inhibiting the telomerase activity and increasing the expression of P53 protein may be one of the most important mechanisms.
2003, 10(3):202-205.
DOI: 10.3872/j.issn.1007-385X.2003.3.011
Abstract:
Objective:To study the anti-tumor effect of recombinant human MUC1-MBP. Methods: The C57BL/6 mice were in oculated with MUC1-MBP by subcutaneous. MUC1 specific CTL activity of spleen were determined by MTT; The effects on prevention and treatment of tumor were observed by establishing lewis lung cancer-carrying mice. Results:The cytotoxicity of CTL from immunized mice to the MCF7 and Lewis lung cancer cells respectively was (47.7±4.3) % and (67.5 ±6.5) %; 5×105 lewis lung cancer cells following immunization were injected iv into C57BL/6 mice, after three weeks, the number of lung and tail tumor colonies was 51 and 5 for PBS and MUC1-MBP groups respectively and the suvival time was significantly delayed in immunized mice. The average volume of tumors in mice with MUC1-MBP was 386 mm3 wherea control group was 4 000 mm3 at tumor treating experiment. Conclusions:Recombinant human MUC1-MBP have significantly effects on prevention, treatment and inhibiting metastases of tumor. Our results suggested that the recombinant MUC1-MBP might be used to develop protein vaccine against human carcinoma.
Objective:To study the anti-tumor effect of recombinant human MUC1-MBP. Methods: The C57BL/6 mice were in oculated with MUC1-MBP by subcutaneous. MUC1 specific CTL activity of spleen were determined by MTT; The effects on prevention and treatment of tumor were observed by establishing lewis lung cancer-carrying mice. Results:The cytotoxicity of CTL from immunized mice to the MCF7 and Lewis lung cancer cells respectively was (47.7±4.3) % and (67.5 ±6.5) %; 5×105 lewis lung cancer cells following immunization were injected iv into C57BL/6 mice, after three weeks, the number of lung and tail tumor colonies was 51 and 5 for PBS and MUC1-MBP groups respectively and the suvival time was significantly delayed in immunized mice. The average volume of tumors in mice with MUC1-MBP was 386 mm3 wherea control group was 4 000 mm3 at tumor treating experiment. Conclusions:Recombinant human MUC1-MBP have significantly effects on prevention, treatment and inhibiting metastases of tumor. Our results suggested that the recombinant MUC1-MBP might be used to develop protein vaccine against human carcinoma.
2003, 10(3):206-209.
DOI: 10.3872/j.issn.1007-385X.2003.3.012
Abstract:
Objective：To investigate the effects of Tea Polyphenol(TP) on inhibition of tumor growth, anti-oxidation and immune regulation in tumor-bearing mice. Methods: TP was administered by gastriclavage to C57BL/6J mice implanted with Lewis lung cancer. Results: Inhibitory rates of TP at doses of 125 mg/kg and 250 mg/kg were 27.2% and 188% respectively, with significance over controls at dose of 125 mg/kg(P<0.05). In tumor-bearing mice, weight of thymus and its index were declined and spleen index was increased, while serum MDA levels were increased significantly at the same time, SOD and GSH-Px activities had no change after mice were beard tumor. Serum MDA levels of tumor-bearing mice were decreased significantly and SOD and GSH-Px activities were increased by TP. Thymus index and spleen index were decreased at the same ime.Conclusion: TP could inhibit the growth of Lewis lung cancer, the underlying mechanism of which was associated with anti-oxidation.
Objective：To investigate the effects of Tea Polyphenol(TP) on inhibition of tumor growth, anti-oxidation and immune regulation in tumor-bearing mice. Methods: TP was administered by gastriclavage to C57BL/6J mice implanted with Lewis lung cancer. Results: Inhibitory rates of TP at doses of 125 mg/kg and 250 mg/kg were 27.2% and 188% respectively, with significance over controls at dose of 125 mg/kg(P<0.05). In tumor-bearing mice, weight of thymus and its index were declined and spleen index was increased, while serum MDA levels were increased significantly at the same time, SOD and GSH-Px activities had no change after mice were beard tumor. Serum MDA levels of tumor-bearing mice were decreased significantly and SOD and GSH-Px activities were increased by TP. Thymus index and spleen index were decreased at the same ime.Conclusion: TP could inhibit the growth of Lewis lung cancer, the underlying mechanism of which was associated with anti-oxidation.
2003, 10(3):210-213.
DOI: 10.3872/j.issn.1007-385X.2003.3.013
Abstract:
Objective:To investigate the effects of AMB(Astragalus Mongholicus Bge) on the immunosuppression of tumor cell line S180 supernatant.Methods:The effects of the supernatant of S180 cultured with or without AMB on the transformation, IL-2 production and NK activity of murine splenocytes and the reactivity of CTLL-2 to IL-2 were measured by MTT, and the effect on the IL-2Rα expression of murine splenocyte was detected by flow cytometry. Results: The supernatant of S180 alone could strongly inhibit the five tests. Adding AMB to the superanant of S180,the results of the five tests were up-regulated substantially.The superanants of S180 which were cultured first in AMB for 24 h or 48 h,then washing off AMB or not,and recultured for 24 h remarkably up-regulated the five tests. Conclusions:AMB could reverse the immunosuppressions of the supernatant of S180,down-regulate the synthesis and/or secretion of immunosuppressive subsance by tumor cells.
Objective:To investigate the effects of AMB(Astragalus Mongholicus Bge) on the immunosuppression of tumor cell line S180 supernatant.Methods:The effects of the supernatant of S180 cultured with or without AMB on the transformation, IL-2 production and NK activity of murine splenocytes and the reactivity of CTLL-2 to IL-2 were measured by MTT, and the effect on the IL-2Rα expression of murine splenocyte was detected by flow cytometry. Results: The supernatant of S180 alone could strongly inhibit the five tests. Adding AMB to the superanant of S180,the results of the five tests were up-regulated substantially.The superanants of S180 which were cultured first in AMB for 24 h or 48 h,then washing off AMB or not,and recultured for 24 h remarkably up-regulated the five tests. Conclusions:AMB could reverse the immunosuppressions of the supernatant of S180,down-regulate the synthesis and/or secretion of immunosuppressive subsance by tumor cells.
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Chinese Journal Of Cancer Biotheray ® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.